Pseudomonas aeruginosa PAO1, PA0044 (exoT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0044
Name
exoT
Replicon chromosome
Genomic location 58786 - 60159 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248734.1
GI 15595242
Affymetrix PA0044_exoT_at
Entrez 878350
GenBank AAG03434.1
INSDC AAG03434.1
NCBI Locus Tag PA0044
protein_id(GenBank) gb|AAG03434.1|AE004444_3|gnl|PseudoCAP|PA0044
TIGR NTL03PA00045
UniParc UPI00000C4EFE
UniProtKB Acc Q9I788
UniProtKB ID Q9I788_PSEAE
UniRef100 UniRef100_Q9I788
UniRef50 UniRef50_Q93Q17
UniRef90 UniRef90_Q9I788

Product

Feature Type CDS
Coding Frame 1
Product Name
exoenzyme T
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -8.32
Kyte-Doolittle Hydrophobicity Value -0.313
Molecular Weight (kDa) 48.5
Isoelectric Point (pI) 5.66

Subcellular localization

Individual Mappings
Localization Confidence PMID
Host Associated
Note: This is based on PSORTb V3.0 secondary localization prediction
 Class 3
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6JNP TOXIN 03/17/19 Structure of ExoT-SpcS Complex from Pseudomonas aeruginosa in 2.2 Angstrom Pseudomonas aeruginosa 2.261 X-RAY DIFFRACTION 98.2
4JMF TOXIN/CHAPERONE 03/14/13 Crystal structure of ExoT (residues 28 -77)- SpcS complex from Pseudomonas aeruginosa at 2.1 angstrom Pseudomonas aeruginosa 2.099 X-RAY DIFFRACTION 100.0
6GNN TOXIN 05/31/18 Exoenzyme T from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form bound to STO1101 Homo sapiens; Pseudomonas aeruginosa PAO1 3.79 X-RAY DIFFRACTION 99.6

Virulence Evidence

Source 1
Database VFDB : VF0097
Category T3SS translocated effectors
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 10603417
Source 2
Database Victors : 4219
Category T3SS translocated effectors
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Cricetulus griseus
Notes exoT interferes with Rho signal transduction pathways, which regulate actin organization, exocytosis, cell cycle progression, and phagocytosis (CHO cells)
Reference 10992524

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 8 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000044 (294 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0044
Search term: exoT
Search term: exoenzyme T

Human Homologs

References

Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon.
Yahr TL, Mende-Mueller LM, Friese MB, Frank DW
J Bacteriol 1997 Nov;179(22):7165-8
PubMed ID: 9371466