Pseudomonas aeruginosa PAO1, PA0080 (tssJ1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0080
Name
tssJ1
Replicon chromosome
Genomic location 97754 - 98218 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_248770.1
GI 15595278
Affymetrix PA0080_at
Entrez 879436
GenBank AAG03470.1
INSDC AAG03470.1
NCBI Locus Tag PA0080
protein_id(GenBank) gb|AAG03470.1|AE004447_3|gnl|PseudoCAP|PA0080
TIGR NTL03PA00081
UniParc UPI00000C4F21
UniProtKB Acc Q9I752
UniProtKB ID Q9I752_PSEAE
UniRef100 UniRef100_W0YJI0
UniRef50 UniRef50_A6UXL2
UniRef90 UniRef90_A6UXL2

Product

Feature Type CDS
Coding Frame 1
Product Name
TssJ1
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected using LC-MS/MS (16.93 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.56
Kyte-Doolittle Hydrophobicity Value -0.173
Molecular Weight (kDa) 16.9
Isoelectric Point (pI) 6.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3ZHN TOXIN 12/22/12 Crystal structure of the T6SS lipoprotein TssJ1 from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.4 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0334
Category Hcp secretion island-1 encoded type VI secretion system (H-T6SS)
Evidence ECO:0000305
curator inference used in manual assertion
Host Organism
Notes
Reference 16763151

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 77 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000079 (812 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0080
Search term: tssJ1
Search term: TssJ1

Human Homologs

References

A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria.
Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD
Cell Host Microbe 2010 Jan 21;7(1):25-37
PubMed ID: 20114026
In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages.
Shalom G, Shaw JG, Thomas MS
Microbiology (Reading) 2007 Aug;153(Pt 8):2689-2699
PubMed ID: 17660433
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344