Pseudomonas aeruginosa PAO1, PA0342 (thyA)

Gene Ontology

Ontology	Accession	Term	GO Evidence	Evidence Ontology (ECO) Code	Reference	Comments
Molecular Function	GO:0004799	thymidylate synthase activity	ISM Inferred from Sequence Model Term mapped from: InterPro:TIGR03284	ECO:0000259 match to InterPro signature evidence used in automatic assertion
Molecular Function	GO:0016741	transferase activity, transferring one-carbon groups	ISM Inferred from Sequence Model Term mapped from: InterPro:PTHR11548	ECO:0000259 match to InterPro signature evidence used in automatic assertion
Biological Process	GO:0006231	dTMP biosynthetic process	ISM Inferred from Sequence Model Term mapped from: InterPro:TIGR03284	ECO:0000259 match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Biosynthesis of cofactors, prosthetic groups and carriers Other PAO1 genes in this class Nucleotide biosynthesis and metabolism Other PAO1 genes in this class

Pathways

Database	Xref	Pathway	Version	Evidence	PMID
PseudoCyc	1CMET2-PWY	N<sup>10</sup>-formyl-tetrahydrofolate biosynthesis	19.5	ECO:0000250 sequence similarity evidence used in manual assertion	12867747
KEGG	pae01100	Metabolic pathways	81.0+/01-23, Jan 17	ECO:0000249 sequence similarity evidence used in automatic assertion
KEGG	pae00670	One carbon pool by folate	81.0+/01-23, Jan 17	ECO:0000249 sequence similarity evidence used in automatic assertion
PseudoCyc	PWY-7187	pyrimidine deoxyribonucleotides de novo biosynthesis II	19.5	ECO:0000250 sequence similarity evidence used in manual assertion	12867747
PseudoCyc	PWY0-166	superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	19.5	ECO:0000250 sequence similarity evidence used in manual assertion	12867747
PseudoCyc	PWY-7184	pyrimidine deoxyribonucleotides de novo biosynthesis I	19.5	ECO:0000250 sequence similarity evidence used in manual assertion	12867747
KEGG	pae00240	Pyrimidine metabolism	81.0+/01-23, Jan 17	ECO:0000249 sequence similarity evidence used in automatic assertion
PseudoCAP		Folate biosynthesis		ECO:0000037 not_recorded
PseudoCAP		One carbon pool by folate		ECO:0000037 not_recorded
PseudoCAP		Pyrimidine metabolism		ECO:0000037 not_recorded

Functional Predictions from Interpro

Analysis	Accession	Description	Interpro Accession	Interpro Description	Amino Acid Start	Amino Acid Stop	E-value
PRINTS	PR00108	Thymidylate synthase family signature	IPR000398	Thymidylate synthase	115	134	9.9E-48
PRINTS	PR00108	Thymidylate synthase family signature	IPR000398	Thymidylate synthase	141	156	9.9E-48
NCBIfam	TIGR03284	JCVI: thymidylate synthase	IPR000398	Thymidylate synthase	84	264	5.3E-96
Pfam	PF00303	Thymidylate synthase	IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	2	264	1.0E-126
PRINTS	PR00108	Thymidylate synthase family signature	IPR000398	Thymidylate synthase	159	185	9.9E-48
CDD	cd00351	TS_Pyrimidine_HMase	IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	3	216	2.09763E-126
PRINTS	PR00108	Thymidylate synthase family signature	IPR000398	Thymidylate synthase	42	63	9.9E-48
Gene3D	G3DSA:3.30.572.10	Thymidylate synthase/dCMP hydroxymethylase domain	IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	1	264	0.0
FunFam	G3DSA:3.30.572.10:FF:000001	Thymidylate synthase	-	-	1	264	0.0
NCBIfam	TIGR03284	JCVI: thymidylate synthase	IPR000398	Thymidylate synthase	2	85	1.4E-40
SUPERFAMILY	SSF55831	Thymidylate synthase/dCMP hydroxymethylase	IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	1	264	0.0
PRINTS	PR00108	Thymidylate synthase family signature	IPR000398	Thymidylate synthase	197	214	9.9E-48
Hamap	MF_00008	Thymidylate synthase [thyA].	IPR000398	Thymidylate synthase	1	264	41.267605
PANTHER	PTHR11548	THYMIDYLATE SYNTHASE 1	IPR045097	Thymidylate synthase/dCMP hydroxymethylase	2	264	1.9E-113

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.