Pseudomonas aeruginosa PAO1, PA0432 (sahH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0432
Name
sahH
Synonym: ahcY
Replicon chromosome
Genomic location 482706 - 484115 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249123.1
GI 15595629
Affymetrix PA0432_sahH_at
Entrez 877746
GenBank AAG03821.1
INSDC AAG03821.1
NCBI Locus Tag PA0432
protein_id(GenBank) gb|AAG03821.1|AE004480_5|gnl|PseudoCAP|PA0432
TIGR NTL03PA00433
UniParc UPI0000135502
UniProtKB Acc Q9I685
UniProtKB ID SAHH_PSEAE
UniRef100 UniRef100_B7V419
UniRef50 UniRef50_Q4K4H7
UniRef90 UniRef90_A4XPF3

Product

Feature Type CDS
Coding Frame 1
Product Name
S-adenosyl-L-homocysteine hydrolase
Product Name Confidence: Class 2
Synonyms adenosylhomocysteinase (adohcyase)
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.34
Kyte-Doolittle Hydrophobicity Value -0.189
Molecular Weight (kDa) 51.4
Isoelectric Point (pI) 5.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6F3M HYDROLASE 11/28/17 Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa complexed with adenosine, K+ and Zn2+ cations PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.6 X-RAY DIFFRACTION 100.0
6F3N HYDROLASE 11/28/17 Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa cocrystallized with SAH in the presence of K+ and Zn2+ cations PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.85 X-RAY DIFFRACTION 100.0
6F3Q HYDROLASE 11/28/17 Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa in complex with adenine and Rb+ cation PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.45 X-RAY DIFFRACTION 100.0
6F3P HYDROLASE 11/28/17 Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa in complex with 3'-deoxyadenosine and K+ cation PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.35 X-RAY DIFFRACTION 100.0
6F3O HYDROLASE 11/28/17 Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa complexed with adenine, K+ and Zn2+ cations PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.75 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 500 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000423 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0432
Search term: sahH

Human Homologs

Ensembl 97, assembly GRCh38.p12
adenosylhomocysteinase [Source:HGNC Symbol;Acc:HGNC:343]
E-value: 1.1e-134
Percent Identity: 56.2

References

Amino acid sequence of S-adenosyl-L-homocysteine hydrolase from rat liver as derived from the cDNA sequence.
Ogawa H, Gomi T, Mueckler MM, Fujioka M, Backlund PS Jr, Aksamit RR, Unson CG, Cantoni GL
Proc Natl Acad Sci U S A 1987 Feb;84(3):719-23
PubMed ID: 3027698
Isozyme and DNA analysis of human S-adenosyl-L-homocysteine hydrolase (AHCY).
Arredondo-Vega FX, Charlton JA, Edwards YH, Hopkinson DA, Whitehouse DB
Ann Hum Genet 1989 May;53(2):157-67
PubMed ID: 2574561
Mutational and nucleotide sequence analysis of S-adenosyl-L-homocysteine hydrolase from Rhodobacter capsulatus.
Sganga MW, Aksamit RR, Cantoni GL, Bauer CE
Proc Natl Acad Sci U S A 1992 Jul 15;89(14):6328-32
PubMed ID: 1631127
S-adenosylhomocysteine hydrolase from Acinetobacter calcoaceticus: purification and partial characterization.
Franceschini N, Impagnatiello A, Oratore A, Strom R, Bozzi A
Biochem. Mol. Biol. Int. 1993 Aug;30(6):1081-92
PubMed ID: 8220254
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994