Pseudomonas aeruginosa PAO1, PA0623

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0623
Name
Replicon chromosome
Genomic location 682567 - 683070 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249314.1
GI 15595820
Affymetrix PA0623_at
DNASU PaCD00006276
Entrez 880639
GenBank AAG04012.1
INSDC AAG04012.1
NCBI Locus Tag PA0623
protein_id(GenBank) gb|AAG04012.1|AE004498_7|gnl|PseudoCAP|PA0623
TIGR NTL03PA00624
UniParc UPI00000C50D9
UniProtKB Acc Q9I5S9
UniProtKB ID Q9I5S9_PSEAE
UniRef100 UniRef100_W0YMC3
UniRef50 UniRef50_Q4KF54
UniRef90 UniRef90_A6UZC6

Product

Feature Type CDS
Coding Frame 1
Product Name
probable bacteriophage protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (17.94 kDa, PMID:21751344)
Charge (pH 7) -3.01
Kyte-Doolittle Hydrophobicity Value -0.106
Molecular Weight (kDa) 17.9
Isoelectric Point (pI) 4.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6U5B ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - precontracted - baseplate Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.5 ELECTRON MICROSCOPY 100.0
5W5E STRUCTURAL PROTEIN 06/15/17 Re-refinement of the pyocin tube structure Pseudomonas aeruginosa 3.5 ELECTRON MICROSCOPY 100.0
6U5F ANTIMICROBIAL PROTEIN 08/27/19 CryoEM Structure of Pyocin R2 - precontracted - collar Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 3.8 ELECTRON MICROSCOPY 100.0
6PYT ANTIMICROBIAL PROTEIN 07/30/19 CryoEM Structure of Pyocin R2 - precontracted - trunk Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.9 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 83 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000606 (315 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0623

Human Homologs

References

Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344