Pseudomonas aeruginosa PAO1, PA0715

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0715
Name
Replicon chromosome
Genomic location 785969 - 786925 (+ strand)
CommentPA0715 inhibition results in decrease in pyocyanin production in strain PAO1 (PMID:36579125)
CommentPA0715 is Required for Motility and Chemotaxis (PMID:36579125)

Cross-References

RefSeq NP_249406.1
GI 15595912
Affymetrix PA0715_at
DNASU PaCD00006328
Entrez 879902
GenBank AAG04104.1
INSDC AAG04104.1
NCBI Locus Tag PA0715
protein_id(GenBank) gb|AAG04104.1|AE004507_3|gnl|PseudoCAP|PA0715
TIGR NTL03PA00716
UniParc UPI00000C5118
UniProtKB Acc Q9I5L3
UniProtKB ID Q9I5L3_PSEAE
UniRef100 UniRef100_Q9I5L3
UniRef50 UniRef50_Q9I5L3
UniRef90 UniRef90_Q9I5L3

Product

Feature Type CDS
Coding Frame 1
Product Name
putative reverse transcriptase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 16.28
Kyte-Doolittle Hydrophobicity Value -0.196
Molecular Weight (kDa) 36.6
Isoelectric Point (pI) 9.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 102 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000692 (11 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0715

Human Homologs

References

Structure and biosynthesis of unbranched multicopy single-stranded DNA by reverse transcriptase in a clinical Escherichia coli isolate.
Lim D
Mol Microbiol 1992 Dec;6(23):3531-42
PubMed ID: 1282191
CRISPRi-Mediated Gene Suppression Reveals Putative Reverse Transcriptase Gene PA0715 to Be a Global Regulator of Pseudomonas aeruginosa.
Zhou D, Huang G, Xu G, Xiang L, Huang S, Chen X, Zhang Y, Wang D
Infect Drug Resist 2022;15:7577-7599
PubMed ID: 36579125