Pseudomonas aeruginosa PAO1, PA0721 (pfsE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0721
Name
pfsE
Replicon chromosome
Genomic location 790617 - 790709 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentPfsE suppresses twitching motility and protects P. aeruginosa from Pf4 superinfection
CommentPfsE transiently inhibits type IV pili (T4P) assembly through an interaction with the T4P platform protein PilC

Cross-References

RefSeq NP_249412.1
GI 15595918
Affymetrix PA0721_r_at
DNASU PaCD00006330
Entrez 880780
GenBank AAG04110.1
INSDC AAG04110.1
NCBI Locus Tag PA0721
protein_id(GenBank) gb|AAG04110.1|AE004507_9|gnl|PseudoCAP|PA0721
TIGR NTL03PA00722
UniParc UPI00000C511E
UniProtKB Acc Q9I5K7
UniProtKB ID Q9I5K7_PSEAE
UniRef100 UniRef100_Q56VP4
UniRef50 UniRef50_P25137
UniRef90 UniRef90_P25137

Product

Feature Type CDS
Coding Frame 1
Product Name
Pf superinfection exclusion, PfsE
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -0.02
Kyte-Doolittle Hydrophobicity Value 1.163
Molecular Weight (kDa) 3.2
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000697 (256 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0721
Search term: pfsE

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
A Filamentous Bacteriophage Protein Inhibits Type IV Pili To Prevent Superinfection of Pseudomonas aeruginosa.
Schmidt AK, Fitzpatrick AD, Schwartzkopf CM, Faith DR, Jennings LK, Coluccio A, Hunt DJ, Michaels LA, Hargil A, Chen Q, Bollyky PL, Dorward DW, Wachter J, Rosa PA, Maxwell KL, Secor PR
mBio 2022 Feb 22;13(1):e0244121
PubMed ID: 35038902