Pseudomonas aeruginosa PAO1, PA0729 (pfiT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0729
Name
pfiT
Replicon chromosome
Genomic location 797251 - 797598 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1

Cross-References

RefSeq NP_249420.1
GI 15595926
Affymetrix PA0729_at
Entrez 877701
GenBank AAG04118.1
INSDC AAG04118.1
NCBI Locus Tag PA0729
protein_id(GenBank) gb|AAG04118.1|AE004508_3|gnl|PseudoCAP|PA0729
TIGR NTL03PA00730
UniParc UPI00000C5125
UniProtKB Acc Q9I5J9
UniProtKB ID Q9I5J9_PSEAE
UniRef100 UniRef100_Q9I5J9
UniRef50 UniRef50_Q48B85
UniRef90 UniRef90_Q9I5J9

Product

Feature Type CDS
Coding Frame 1
Product Name
Pf4 inhibition toxin, PfiT
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.32
Kyte-Doolittle Hydrophobicity Value -0.085
Molecular Weight (kDa) 13.0
Isoelectric Point (pI) 4.42

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 9 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000705 (93 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0729
Search term: pfiT

Human Homologs

References

Complete DNA sequence and detailed analysis of the Yersinia pestis KIM5 plasmid encoding murine toxin and capsular antigen.
Lindler LE, Plano GV, Burland V, Mayhew GF, Blattner FR
Infect Immun 1998 Dec;66(12):5731-42
PubMed ID: 9826348
Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa.
Li Y, Liu X, Tang K, Wang W, Guo Y, Wang X
Microb Biotechnol 2020 Jul;13(4):1132-1144
PubMed ID: 32246813