Pseudomonas aeruginosa PAO1, PA0761 (nadB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0761
Name
nadB
Replicon chromosome
Genomic location 829276 - 830892 (- strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
CommentReaction not utilizing O2 is necessary to produce spermidine in anaerobic conditions, as shown by gap analysis and explained in references.

Cross-References

RefSeq NP_249452.1
GI 15595958
Affymetrix PA0761_nadB_at
Entrez 882067
GenBank AAG04150.1
INSDC AAA64438.1
NCBI Locus Tag PA0761
protein_id(GenBank) gb|AAG04150.1|AE004511_3|gnl|PseudoCAP|PA0761
TIGR NTL03PA00762
UniParc UPI000012FC85
UniProtKB Acc Q51363
UniProtKB ID NADB_PSEAE
UniRef100 UniRef100_Q51363
UniRef50 UniRef50_P10902
UniRef90 UniRef90_Q51363

Product

Feature Type CDS
Coding Frame 1
Product Name
L-aspartate oxidase
Product Name Confidence: Class 2
Synonyms L-aspartate oxidase(aerobic/anaerobic) NadB
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.69
Kyte-Doolittle Hydrophobicity Value -0.248
Molecular Weight (kDa) 60.0
Isoelectric Point (pI) 6.26

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 609 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000735 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0761
Search term: nadB

Human Homologs

Ensembl 97, assembly GRCh38.p12
succinate dehydrogenase complex flavoprotein subunit A [Source:HGNC Symbol;Acc:HGNC:10680]
E-value: 1.7e-62
Percent Identity: 32.7

References

Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in nadB does not affect NAD biosynthesis or alginate production.
DeVries CA, Hassett DJ, Flynn JL, Ohman DE
Gene 1995 Apr;156(1):63-7
PubMed ID: 7737518
Nicotinamide adenine dinucleotide biosynthesis and pyridine nucleotide cycle metabolism in microbial systems.
Foster JW, Moat AG
Microbiol. Rev. 1980 Mar;44(1):83-105
PubMed ID: 6997723