Pseudomonas aeruginosa PAO1, PA4560 (ileS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Ontology

Ontology Accession Term GO Evidence Evidence Ontology (ECO) Code Reference Comments
Biological Process GO:0044267 cellular protein metabolic process
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000044
sequence similarity evidence
Biological Process GO:0006520 cellular amino acid metabolic process
ISS
Inferred from Sequence or Structural Similarity 		ECO:0000044
sequence similarity evidence
Molecular Function GO:0005524 ATP binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF00133
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0000166 nucleotide binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF00133
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0000049 tRNA binding
ISM
Inferred from Sequence Model
Term mapped from: InterPro:cd07960
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Biological Process GO:0006428 isoleucyl-tRNA aminoacylation
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PR00984
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Biological Process GO:0006418 tRNA aminoacylation for protein translation
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF00133
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0002161 aminoacyl-tRNA editing activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:SSF50677
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0004822 isoleucine-tRNA ligase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:MF_02002
 ECO:0000259
match to InterPro signature evidence used in automatic assertion
Molecular Function GO:0004812 aminoacyl-tRNA ligase activity
ISM
Inferred from Sequence Model
Term mapped from: InterPro:PF00133
 ECO:0000259
match to InterPro signature evidence used in automatic assertion

Functional Classifications Manually Assigned by PseudoCAP

Amino acid biosynthesis and metabolism Other PAO1 genes in this class
Translation, post-translational modification, degradation Other PAO1 genes in this class

Pathways

Database Xref Pathway Version Evidence PMID
PseudoCyc TRNA-CHARGING-PWY tRNA charging 19.5 ECO:0000250
sequence similarity evidence used in manual assertion 		12867747
PseudoCAP Valine, leucine and isoleucine biosynthesis ECO:0000037
not_recorded
PseudoCAP Aminoacyl-tRNA biosynthesis ECO:0000037
not_recorded
KEGG pae00970 Aminoacyl-tRNA biosynthesis 81.0+/01-23, Jan 17 ECO:0000249
sequence similarity evidence used in automatic assertion

Functional Predictions from Interpro

Analysis Accession Description Interpro Accession Interpro Description Amino Acid Start Amino Acid Stop E-value
Pfam PF00133 tRNA synthetases class I (I, L, M and V) IPR002300 Aminoacyl-tRNA synthetase, class Ia 28 646 0.0
CDD cd00818 IleRS_core - - 49 643 4.82756E-98
Hamap MF_02002 Isoleucine--tRNA ligase [ileS]. IPR023585 Isoleucine-tRNA ligase, type 1 2 934 21.214516
FunFam G3DSA:3.40.50.620:FF:000048 Isoleucine--tRNA ligase - - 377 644 2.7E-126
PRINTS PR00984 Isoleucyl-tRNA synthetase signature IPR002301 Isoleucine-tRNA ligase 399 414 3.1E-32
Gene3D G3DSA:1.10.10.830 - - - 480 532 1.2E-5
Gene3D G3DSA:3.40.50.620 HUPs IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 16 210 8.6E-51
PRINTS PR00984 Isoleucyl-tRNA synthetase signature IPR002301 Isoleucine-tRNA ligase 235 258 3.1E-32
PRINTS PR00984 Isoleucyl-tRNA synthetase signature IPR002301 Isoleucine-tRNA ligase 529 542 3.1E-32
FunFam G3DSA:3.40.50.620:FF:000042 Isoleucine--tRNA ligase - - 18 211 1.0E-97
Pfam PF06827 Zinc finger found in FPG and IleRS IPR010663 Zinc finger, FPG/IleRS-type 905 931 1.0E-7
SUPERFAMILY SSF50677 ValRS/IleRS/LeuRS editing domain IPR009008 Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain 201 401 5.76E-54
CDD cd07960 Anticodon_Ia_Ile_BEm IPR033708 Isoleucyl tRNA synthetase type 1, anticodon-binding domain 646 826 3.25413E-84
NCBIfam TIGR00392 JCVI: isoleucine--tRNA ligase IPR002301 Isoleucine-tRNA ligase 15 849 0.0
SUPERFAMILY SSF47323 Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases IPR009080 Aminoacyl-tRNA synthetase, class Ia, anticodon-binding 658 934 2.24E-77
Gene3D G3DSA:3.40.50.620 HUPs IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 377 646 2.9E-66
Pfam PF08264 Anticodon-binding domain of tRNA ligase IPR013155 Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding 691 847 1.3E-27
PRINTS PR00984 Isoleucyl-tRNA synthetase signature IPR002301 Isoleucine-tRNA ligase 51 62 3.1E-32
FunFam G3DSA:1.10.730.20:FF:000001 Isoleucine--tRNA ligase - - 647 935 4.4E-118
PANTHER PTHR42765 SOLEUCYL-TRNA SYNTHETASE - - 3 937 0.0
PRINTS PR00984 Isoleucyl-tRNA synthetase signature IPR002301 Isoleucine-tRNA ligase 567 576 3.1E-32
Gene3D G3DSA:1.10.730.20 - - - 647 935 2.1E-92
SUPERFAMILY SSF52374 Nucleotidylyl transferase - - 3 652 0.0

Search for additional functional domains at the NCBI CDD database website. Go to this protein's amino acid sequence and follow the link.