Pseudomonas aeruginosa PAO1, PA5396

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5396
Name
Replicon chromosome
Genomic location 6074230 - 6075207 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254083.1
GI 15600589
Affymetrix PA5396_at
Entrez 878120
GenBank AAG08781.1
INSDC AAG08781.1
NCBI Locus Tag PA5396
protein_id(GenBank) gb|AAG08781.1|AE004952_3|gnl|PseudoCAP|PA5396
TIGR NTL03PA05397
UniParc UPI00000C5FE1
UniProtKB Acc Q9HTG8
UniProtKB ID Q9HTG8_PSEAE
UniRef100 UniRef100_Q9HTG8
UniRef50 UniRef50_I1EZS2
UniRef90 UniRef90_G8Q2F1

Product

Feature Type CDS
Coding Frame 1
Product Name
amidohydrolase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Charge (pH 7) -8.01
Kyte-Doolittle Hydrophobicity Value -0.207
Molecular Weight (kDa) 36.1
Isoelectric Point (pI) 5.43

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2I5G HYDROLASE 08/24/06 Crystal strcuture of amidohydrolase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 99.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 276 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004953 (510 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5396
Search term: amidohydrolase

Human Homologs

Ensembl 110, assembly GRCh38.p14
dipeptidase 2 [Source:HGNC Symbol;Acc:HGNC:23028]
E-value: 3.2e-23
Percent Identity: 25.8
Ensembl 110, assembly GRCh38.p14
dipeptidase 2 [Source:HGNC Symbol;Acc:HGNC:23028]
E-value: 3.2e-23
Percent Identity: 25.8

References

Predicting drug targets by homology modelling of Pseudomonas aeruginosa proteins of unknown function.
Babic N, Kovacic F
PLoS One 2021;16(10):e0258385
PubMed ID: 34648550