Pseudomonas aeruginosa PAO1, PA5418 (soxA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA5418
Name
soxA
Replicon chromosome
Genomic location 6097024 - 6100041 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentNMR analysis shows buildup of labeled sarcosine when soxA transposon mutants are given labeled choline.
CommentStrains with transposon mutants in any of the soxBDAG genes are unable to grow on choline, glycine betaine, dimethylglcycine or sarcosine.

Cross-References

RefSeq NP_254105.1
GI 15600611
Affymetrix PA5418_soxA_at
Entrez 880824
GenBank AAG08803.1
INSDC AAG08803.1
NCBI Locus Tag PA5418
protein_id(GenBank) gb|AAG08803.1|AE004954_5|gnl|PseudoCAP|PA5418
TIGR NTL03PA05419
UniParc UPI00000C5FF5
UniProtKB Acc Q9HTE6
UniProtKB ID Q9HTE6_PSEAE
UniRef100 UniRef100_Q9HTE6
UniRef50 UniRef50_Q9HTE6
UniRef90 UniRef90_Q9HTE6

Product

Feature Type CDS
Coding Frame 1
Product Name
sarcosine oxidase alpha subunit
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) 4.76
Kyte-Doolittle Hydrophobicity Value -0.236
Molecular Weight (kDa) 109.4
Isoelectric Point (pI) 7.53

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 498 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004972 (528 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5418
Search term: soxA

References

Sequence analysis of sarcosine oxidase and nearby genes reveals homologies with key enzymes of folate one-carbon metabolism.
Chlumsky LJ, Zhang L, Jorns MS
J. Biol. Chem. 1995 Aug;270(31):18252-9
PubMed ID: 7543100
Identification of two gene clusters and a transcriptional regulator required for Pseudomonas aeruginosa glycine betaine catabolism.
Wargo MJ, Szwergold BS, Hogan DA
J. Bacteriol. 2008 Apr;190(8):2690-9
PubMed ID: 17951379