Pseudomonas aeruginosa PAO1, PA5554 (atpD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5554
Name
atpD
Synonym: papB
Synonym: uncD
Replicon chromosome
Genomic location 6248278 - 6249654 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_254241.1
GI 15600747
Affymetrix PA5554_atpD_at
Entrez 878083
GenBank AAG08939.1
INSDC AAG08939.1
NCBI Locus Tag PA5554
protein_id(GenBank) gb|AAG08939.1|AE004967_10|gnl|PseudoCAP|PA5554
TIGR NTL03PA05555
UniParc UPI00000C606E
UniProtKB Acc Q9HT20
UniProtKB ID ATPB_PSEAE
UniRef100 UniRef100_A6VF32
UniRef50 UniRef50_P50002
UniRef90 UniRef90_Q87TT4

Product

Feature Type CDS
Coding Frame 1
Product Name
ATP synthase beta chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (49.50 kDa, PMID:21751344)
Charge (pH 7) -14.57
Kyte-Doolittle Hydrophobicity Value -0.061
Molecular Weight (kDa) 49.5
Isoelectric Point (pI) 4.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 666 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005085 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5554
Search term: atpD

Human Homologs

Ensembl 110, assembly GRCh38.p14
ATP synthase F1 subunit beta [Source:HGNC Symbol;Acc:HGNC:830]
E-value: 4.4e-180
Percent Identity: 70.6

References

The atp operon: nucleotide sequence of the genes for the gamma, beta, and epsilon subunits of Escherichia coli ATP synthase.
Saraste M, Gay NJ, Eberle A, Runswick MJ, Walker JE
Nucleic Acids Res 1981 Oct 24;9(20):5287-96
PubMed ID: 6272217
Mutations in Ser174 and the glycine-rich sequence (Gly149, Gly150, and Thr156) in the beta subunit of Escherichia coli H(+)-ATPase.
Iwamoto A, Omote H, Hanada H, Tomioka N, Itai A, Maeda M, Futai M
J Biol Chem 1991 Sep 5;266(25):16350-5
PubMed ID: 1832155
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344