Pseudomonas aeruginosa PAO1, PA0002 (dnaN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0002
Name
dnaN
Replicon chromosome
Genomic location 2056 - 3159 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_064722.1
GI 15595200
Affymetrix PA0002_dnaN_at
DNASU PaCD00006004
Entrez 879244
GenBank AAG03392.1
INSDC AAG03392.1
NCBI Locus Tag PA0002
protein_id(GenBank) gb|AAG03392.1|AE004440_2|gnl|PseudoCAP|PA0002
TIGR NTL03PA00003
UniParc UPI0000129706
UniProtKB Acc Q9I7C4
UniProtKB ID DPO3B_PSEAE
UniRef100 UniRef100_Q9I7C4
UniRef50 UniRef50_Q9I7C4
UniRef90 UniRef90_Q9I7C4

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, beta chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.60
Kyte-Doolittle Hydrophobicity Value -0.147
Molecular Weight (kDa) 40.7
Isoelectric Point (pI) 4.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4TR8 DNA BINDING PROTEIN 06/15/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA HB15 1.8 X-RAY DIFFRACTION 100.0
6AMS TRANSFERASE 08/11/17 Crystal structure of the DNA polymerase III subunit beta from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PR 2.39 X-RAY DIFFRACTION 100.0
4TSZ TRANSFERASE 06/19/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa with ligand PSEUDOMONAS AERUGINOSA; SYNTHETIC CONSTRUCT 2.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000002 (555 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0002
Search term: dnaN

References

The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.
Burgers PM, Kornberg A, Sakakibara Y
Proc. Natl. Acad. Sci. U.S.A. 1981 Sep;78(9):5391-5
PubMed ID: 6458041
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y
Gene 1984 May;28(2):159-70
PubMed ID: 6234204
Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
Kong XP, Onrust R, O'Donnell M, Kuriyan J
Cell 1992 May;69(3):425-37
PubMed ID: 1349852