Pseudomonas aeruginosa PAO1, PA0124

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0124
Name
Replicon chromosome
Genomic location 143567 - 143848 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentParDE toxin_antitoxin system

Cross-References

RefSeq NP_248814.1
GI 15595322
Affymetrix PA0124_at
DNASU PaCD00006056
Entrez 879205
GenBank AAG03514.1
INSDC AAG03514.1
NCBI Locus Tag PA0124
protein_id(GenBank) gb|AAG03514.1|AE004450_9|gnl|PseudoCAP|PA0124
TIGR NTL03PA00125
UniParc UPI00000C4F4C
UniProtKB Acc Q9I708
UniProtKB ID Q9I708_PSEAE
UniRef100 UniRef100_W0YM54
UniRef50 UniRef50_A6UXR1
UniRef90 UniRef90_A6UXR1

Product

Feature Type CDS
Coding Frame 1
Product Name
ParE toxin
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.22
Kyte-Doolittle Hydrophobicity Value -0.217
Molecular Weight (kDa) 10.5
Isoelectric Point (pI) 10.05

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6XRW TOXIN/ANTITOXIN 07/13/20 Chromosomal ParDE TA system from P. aeruginosa Pseudomonas aeruginosa 1.77 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 14 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000122 (307 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0124
Search term: ParE toxin

Human Homologs

References

The toxin from a ParDE toxin-antitoxin system found in Pseudomonas aeruginosa offers protection to cells challenged with anti-gyrase antibiotics.
Muthuramalingam M, White JC, Murphy T, Ames JR, Bourne CR
Mol Microbiol 2019 Feb;111(2):441-454
PubMed ID: 30427086