Pseudomonas aeruginosa PAO1, PA0157 (triB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0157
Name
triB
Synonym: triB
Replicon chromosome
Genomic location 178455 - 179525 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248847.1
GI 15595355
Affymetrix PA0157_at
DNASU PaCD00006068
Entrez 882134
GenBank AAG03547.1
INSDC AAG03547.1
NCBI Locus Tag PA0157
protein_id(GenBank) gb|AAG03547.1|AE004453_10|gnl|PseudoCAP|PA0157
TIGR NTL03PA00158
UniParc UPI00000C4F6C
UniProtKB Acc Q9I6X5
UniProtKB ID Q9I6X5_PSEAE
UniRef100 UniRef100_A0A077JP41
UniRef50 UniRef50_G8PZD9
UniRef90 UniRef90_W0YL24

Product

Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriB
Product Name Confidence: Class 1
Synonyms probable RND efflux membrane fusion protein precursor
Resistance-Nodulation-Cell Division (RND) triclosan efflux membrane fusion protein, TriB efflux membrane fusion protein precursor)
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.22
Kyte-Doolittle Hydrophobicity Value -0.241
Molecular Weight (kDa) 38.3
Isoelectric Point (pI) 9.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
TriB TriB is a membrane protein that is fused to TriA and both are required for the triclosan efflux pump function of TriABC-OpmH in P. aeruginosa. ARO:3003680 protein homolog model Perfect 667.5 100.0 3.0.1

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 416 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000155 (505 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0157
Search term: triB

References

Identification and characterization of TriABC-OpmH, a triclosan efflux pump of Pseudomonas aeruginosa requiring two membrane fusion proteins.
Mima T, Joshi S, Gomez-Escalada M, Schweizer HP
J. Bacteriol. 2007 Nov;189(21):7600-9
PubMed ID: 17720796