Pseudomonas aeruginosa PAO1, PA0277

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0277
Name
Replicon chromosome
Genomic location 311451 - 312209 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 3C37: putative peptidase Zn-dependent from Geobacter sulfurreducens (root-mean-square deviation: 1.5 angstroms)

Cross-References

RefSeq NP_248968.1
GI 15595474
Affymetrix PA0277_at
Entrez 883050
GenBank AAG03666.1
INSDC AAG03666.1
NCBI Locus Tag PA0277
protein_id(GenBank) gb|AAG03666.1|AE004465_12|gnl|PseudoCAP|PA0277
TIGR NTL03PA00278
UniParc UPI00000C4FD9
UniProtKB Acc Q9I6L3
UniProtKB ID Q9I6L3_PSEAE
UniRef100 UniRef100_Q9I6L3
UniRef50 UniRef50_A0A084ZNG7
UniRef90 UniRef90_A0A0A8R9J7

Product

Feature Type CDS
Coding Frame 1
Product Name
putative Zn-dependent peptidase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (26.63 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -1.68
Kyte-Doolittle Hydrophobicity Value -0.226
Molecular Weight (kDa) 26.6
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Outer Membrane Vesicle Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 318 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000274 (306 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0277

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799