Pseudomonas aeruginosa PAO1, PA0317

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA0317
Name
Replicon	chromosome
Genomic location	356681 - 358075 (+ strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	PaD2HGDH is active with cobalt, manganese, nickel, or cadmium as an alternative metal
Comment	The FAD serves as the reaction center for hydride transfer
Comment	PA0317 is a D-2-hydroxyglutarate dehydrogenase that converts D-2-hydroxyglutarate to 2-ketoglutarate and its alternative substrate D-malate to oxaloacetate
Comment	Zinc and FAD-dependent enzyme whose electron acceptor is unknown and not oxygen. It uses 1 mole of zinc per mole of protein to orient and activate the substrate upon binding

Cross-References

RefSeq	NP_249008.1
GI	15595514
Affymetrix	PA0317_at
Entrez	878323
GenBank	AAG03706.1
INSDC	AAG03706.1
NCBI Locus Tag	PA0317
protein_id(GenBank)	gb|AAG03706.1|AE004469_11|gnl|PseudoCAP|PA0317
TIGR	NTL03PA00318
UniParc	UPI00000C5000
UniProtKB Acc	Q9I6H4
UniProtKB ID	Q9I6H4_PSEAE
UniRef100	UniRef100_Q9I6H4
UniRef50	UniRef50_F5SNV8
UniRef90	UniRef90_Q9I6H4

Product

Feature Type	CDS
Coding Frame	1
Product Name	D-2-hydroxyglutarate dehydrogenase Product Name Confidence: Class 1
Synonyms	D2HGDH
Evidence for Translation	Detected by MALDI-TOF/TOF (PMID:19333994). Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-13.18
Kyte-Doolittle Hydrophobicity Value	-0.079
Molecular Weight (kDa)	51.3
Isoelectric Point (pI)	4.88

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 549 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000314 (528 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0317 Search term: D-2-hydroxyglutarate dehydrogenase

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000315351 D-2-hydroxyglutarate dehydrogenase [Source:HGNC Symbol;Acc:HGNC:28358] E-value: 5.3e-72 Percent Identity: 34.4

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

Coupling between d-3-phosphoglycerate dehydrogenase and d-2-hydroxyglutarate dehydrogenase drives bacterial l-serine synthesis. Zhang W, Zhang M, Gao C, Zhang Y, Ge Y, Guo S, Guo X, Zhou Z, Liu Q, Zhang Y, Ma C, Tao F, Xu P Proc Natl Acad Sci U S A 2017 Sep 5;114(36):E7574-E7582 PubMed ID: 28827360

Kinetic and Bioinformatic Characterization of d-2-Hydroxyglutarate Dehydrogenase from Pseudomonas aeruginosa PAO1. Quaye JA, Gadda G Biochemistry 2020 Dec 29;59(51):4833-4844 PubMed ID: 33301690

Uncovering Zn(2+) as a cofactor of FAD-dependent Pseudomonas aeruginosa PAO1 d-2-hydroxyglutarate dehydrogenase. Quaye JA, Gadda G J Biol Chem 2023 Mar;299(3):103007 PubMed ID: 36775126

The Pseudomonas aeruginosa PAO1 metallo flavoprotein d-2-hydroxyglutarate dehydrogenase requires Zn(2+) for substrate orientation and activation. Quaye JA, Gadda G J Biol Chem 2023 Mar;299(3):103008 PubMed ID: 36775127