Pseudomonas aeruginosa PAO1, PA0355 (pfpI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0355
Name
pfpI
Replicon chromosome
Genomic location 399493 - 400032 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_249046.1
GI 15595552
Affymetrix PA0355_pfpI_at
Entrez 879351
GenBank AAG03744.1
INSDC AAG03744.1
NCBI Locus Tag PA0355
protein_id(GenBank) gb|AAG03744.1|AE004473_6|gnl|PseudoCAP|PA0355
TIGR NTL03PA00356
UniParc UPI00000C5022
UniProtKB Acc Q9I6D8
UniProtKB ID Q9I6D8_PSEAE
UniRef100 UniRef100_W0YMU7
UniRef50 UniRef50_Q48CJ9
UniRef90 UniRef90_A6UYF8

Product

Feature Type CDS
Coding Frame 1
Product Name
protease PfpI
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.83
Kyte-Doolittle Hydrophobicity Value -0.147
Molecular Weight (kDa) 19.2
Isoelectric Point (pI) 5.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 432 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000346 (1249 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0355
Search term: pfpI
Search term: protease PfpI

References

Sequence, expression in Escherichia coli, and analysis of the gene encoding a novel intracellular protease (PfpI) from the hyperthermophilic archaeon Pyrococcus furiosus.
Halio SB, Blumentals II, Short SA, Merrill BM, Kelly RM
J. Bacteriol. 1996 May;178(9):2605-12
PubMed ID: 8626329
Role of intracellular proteases in the antibiotic resistance, motility, and biofilm formation of Pseudomonas aeruginosa.
Fernández L, Breidenstein EB, Song D, Hancock RE
Antimicrob. Agents Chemother. 2012 Feb;56(2):1128-32
PubMed ID: 22123702