Pseudomonas aeruginosa PAO1, PA0447 (gcdH)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0447
Name
gcdH
Replicon chromosome
Genomic location 502719 - 503900 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249138.1
GI 15595644
Affymetrix PA0447_gcdH_at
DNASU PaCD00006187
Entrez 880534
GenBank AAG03836.1
INSDC AAG03836.1
NCBI Locus Tag PA0447
protein_id(GenBank) gb|AAG03836.1|AE004482_3|gnl|PseudoCAP|PA0447
TIGR NTL03PA00448
UniParc UPI00000C505D
UniProtKB Acc Q9I671
UniProtKB ID Q9I671_PSEAE
UniRef100 UniRef100_Q9I671
UniRef50 UniRef50_Q92947
UniRef90 UniRef90_Q4KKG4

Product

Feature Type CDS
Coding Frame 1
Product Name
glutaryl-CoA dehydrogenase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -4.25
Kyte-Doolittle Hydrophobicity Value -0.212
Molecular Weight (kDa) 43.3
Isoelectric Point (pI) 6.12

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 472 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000438 (545 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0447
Search term: gcdH

Human Homologs

Ensembl 94, assembly GRCh38.p12
glutaryl-CoA dehydrogenase [Source:HGNC Symbol;Acc:HGNC:4189]
E-value: 2.1e-147
Percent Identity: 65.1

References

Cloning of glutaryl-CoA dehydrogenase cDNA, and expression of wild type and mutant enzymes in Escherichia coli.
Goodman SI, Kratz LE, DiGiulio KA, Biery BJ, Goodman KE, Isaya G, Frerman FE
Hum. Mol. Genet. 1995 Sep;4(9):1493-8
PubMed ID: 8541831
Glutaric aciduria type I in the Arab and Jewish communities in Israel.
Anikster Y, Shaag A, Joseph A, Mandel H, Ben-Zeev B, Christensen E, Elpeleg ON
Am. J. Hum. Genet. 1996 Nov;59(5):1012-8
PubMed ID: 8900228