Pseudomonas aeruginosa PAO1, PA0509 (nirN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0509
Name
nirN
Replicon chromosome
Genomic location 571111 - 572592 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249200.1
GI 15595706
Affymetrix PA0509_nirN_at
Entrez 878428
GenBank AAG03898.1
INSDC AAG03898.1
NCBI Locus Tag PA0509
protein_id(GenBank) gb|AAG03898.1|AE004488_2|gnl|PseudoCAP|PA0509
TIGR NTL03PA00510
UniParc UPI00000C5098
UniProtKB Acc Q9I609
UniProtKB ID Q9I609_PSEAE
UniRef100 UniRef100_W0YNA8
UniRef50 UniRef50_F8GTF5
UniRef90 UniRef90_A6UYX0

Product

Feature Type CDS
Coding Frame 1
Product Name
NirN
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.79
Kyte-Doolittle Hydrophobicity Value -0.203
Molecular Weight (kDa) 54.0
Isoelectric Point (pI) 7.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown (This protein may have multiple localization sites) Class 3
Periplasmic Class 1
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 57 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000494 (728 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0509
Search term: nirN
Search term: NirN

References

Two c-type cytochromes, NirM and NirC, encoded in the nir gene cluster of Pseudomonas aeruginosa act as electron donors for nitrite reductase.
Hasegawa N, Arai H, Igarashi Y
Biochem. Biophys. Res. Commun. 2001 Nov;288(5):1223-30
PubMed ID: 11700043
Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis.
Kawasaki S, Arai H, Kodama T, Igarashi Y
J. Bacteriol. 1997 Jan;179(1):235-42
PubMed ID: 8982003
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
NirN protein from Pseudomonas aeruginosa is a novel electron-bifurcating dehydrogenase catalyzing the last step of heme d1 biosynthesis.
Adamczack J, Hoffmann M, Papke U, Haufschildt K, Nicke T, Bröring M, Sezer M, Weimar R, Kuhlmann U, Hildebrandt P, Layer G
J. Biol. Chem. 2014 Oct;289(44):30753-62
PubMed ID: 25204657