Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA0577
|
Name |
dnaG
|
Replicon | chromosome |
Genomic location | 636304 - 638298 (- strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
RefSeq | NP_249268.1 |
GI | 15595774 |
Affymetrix | PA0577_dnaG_at |
Entrez | 882047 |
GenBank | AAG03966.1 |
INSDC | AAG03966.1 |
NCBI Locus Tag | PA0577 |
protein_id(GenBank) | gb|AAG03966.1|AE004494_2|gnl|PseudoCAP|PA0577 |
TIGR | NTL03PA00578 |
UniParc | UPI00001321E8 |
UniProtKB Acc | Q9I5W0 |
UniProtKB ID | DNAG_PSEAE |
UniRef100 | UniRef100_Q9I5W0 |
UniRef50 | UniRef50_Q9I5W0 |
UniRef90 | UniRef90_Q9I5W0 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
DNA primase
|
Synonyms | |
Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
Charge (pH 7) | 1.74 |
Kyte-Doolittle Hydrophobicity Value | -0.548 |
Molecular Weight (kDa) | 74.2 |
Isoelectric Point (pI) | 7.27 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5VAZ | TRANSFERASE | 03/28/17 | Crystal structure of a DNA primase domain from Pseudomonas aeruginosa | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 2.4 | X-RAY DIFFRACTION | 100.0 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 590 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000560 (536 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA0577
Search term: dnaG
Search term: DNA primase
|
The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12.
Burton ZF, Gross CA, Watanabe KK, Burgess RR
Cell 1983 Feb;32(2):335-49
PubMed ID: 6186393
|
Enriched sources of Escherichia coli replication proteins. The dnaG primase is a zinc metalloprotein.
Stamford NP, Lilley PE, Dixon NE
Biochim Biophys Acta 1992 Aug 17;1132(1):17-25
PubMed ID: 1511009
|
Cloning and analysis of the dnaG gene encoding Pseudomonas putida DNA primase.
Szafranski P, Smith CL, Cantor CR
Biochim Biophys Acta 1997 Jun 26;1352(3):243-8
PubMed ID: 9224947
|