Pseudomonas aeruginosa PAO1, PA0595 (lptD)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0595
Name
lptD
Synonym: imp
Synonym: ostA
Replicon chromosome
Genomic location 653753 - 656527 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Comment
CommentLocalication to outer membrane vesicle confirmed in three separate studies (PMID:22909304, PMID:21751344 and PMID:26303878).

Cross-References

RefSeq NP_249286.1
GI 15595792
Affymetrix PA0595_ostA_at
Entrez 880756
GenBank AAG03984.1
INSDC AAG03984.1
NCBI Locus Tag PA0595
protein_id(GenBank) gb|AAG03984.1|AE004495_8|gnl|PseudoCAP|PA0595
TIGR NTL03PA00596
UniParc UPI0000130ED1
UniProtKB Acc Q9I5U2
UniProtKB ID LPTD_PSEAE
UniRef100 UniRef100_Q9I5U2
UniRef50 UniRef50_Q88A43
UniRef90 UniRef90_Q9I5U2

Product

Feature Type CDS
Coding Frame 1
Product Name
LPS-assembly protein LptD
Product Name Confidence: Class 1
Synonyms organic solvent tolerance protein OstA precursor
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (104.27 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.50
Kyte-Doolittle Hydrophobicity Value -0.599
Molecular Weight (kDa) 104.3
Isoelectric Point (pI) 5.29

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5IVA TRANSPORT PROTEIN 03/20/16 The LPS Transporter LptDE from Pseudomonas aeruginosa, core complex Pseudomonas aeruginosa 2.988 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 254 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000578 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0595
Search term: lptD

Human Homologs

References

Cloning of organic solvent tolerance gene ostA that determines n-hexane tolerance level in Escherichia coli.
Aono R, Negishi T, Nakajima H
Appl Environ Microbiol 1994 Dec;60(12):4624-6
PubMed ID: 7811102
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Peptidomimetic antibiotics target outer-membrane biogenesis in Pseudomonas aeruginosa.
Srinivas N, Jetter P, Ueberbacher BJ, Werneburg M, Zerbe K, Steinmann J, Van der Meijden B, Bernardini F, Lederer A, Dias RL, Misson PE, Henze H, Zumbrunn J, Gombert FO, Obrecht D, Hunziker P, Schauer S, Ziegler U, Käch A, Eberl L, Riedel K, DeMarco SJ, Robinson JA
Science 2010 Feb 19;327(5968):1010-3
PubMed ID: 20167788
Outer-membrane protein LptD (PA0595) plays a role in the regulation of alginate synthesis in Pseudomonas aeruginosa.
Pandey S, Delgado C, Kumari H, Florez L, Mathee K
J Med Microbiol 2018 Aug;67(8):1139-1156
PubMed ID: 29923820
Identification of proteins associated with the Pseudomonas aeruginosa biofilm extracellular matrix.
Toyofuku M, Roschitzki B, Riedel K, Eberl L
J Proteome Res 2012 Oct 5;11(10):4906-15
PubMed ID: 22909304
Proteome Profiles of Outer Membrane Vesicles and Extracellular Matrix of Pseudomonas aeruginosa Biofilms.
Couto N, Schooling SR, Dutcher JR, Barber J
J Proteome Res 2015 Oct 2;14(10):4207-22
PubMed ID: 26303878