Pseudomonas aeruginosa PAO1, PA0690 (pdtA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0690
Name
pdtA
Replicon chromosome
Genomic location 749957 - 762499 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249381.1
GI 15595887
Affymetrix PA0690_at
Entrez 880803
GenBank AAG04079.1
INSDC AAG04079.1
NCBI Locus Tag PA0690
protein_id(GenBank) gb|AAG04079.1|AE004504_3|gnl|PseudoCAP|PA0690
TIGR NTL03PA00691
UniParc UPI0000110216
UniProtKB Acc Q9I5N6
UniProtKB ID Q9I5N6_PSEAE
UniRef100 UniRef100_Q9I5N6
UniRef50 UniRef50_Q9I5N6
UniRef90 UniRef90_Q9I5N6

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphate depletion regulated TPS partner A, PdtA
Product Name Confidence: Class 1
Synonyms PdtA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -124.40
Kyte-Doolittle Hydrophobicity Value -0.096
Molecular Weight (kDa) 430.0
Isoelectric Point (pI) 4.48

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence
Outer Membrane Class 1
cell fractionation evidence
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database PseudoCAP
Category two-partner secretion (TPS) system
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Caenorhabditis elegans
Notes PdtA is attenuated for virulence in C. elegans slow killing assay
Infection model Caenorhabditis elegans slow killing assay
Reference 25009238

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 85 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000669 (316 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0690
Search term: pdtA

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Genetic characterization of Bordetella pertussis filamentous haemagglutinin: a protein processed from an unusually large precursor.
Domenighini M, Relman D, Capiau C, Falkow S, Prugnola A, Scarlato V, Rappuoli R
Mol. Microbiol. 1990 May;4(5):787-800
PubMed ID: 2388559
Cloning, expression, and DNA sequence analysis of genes encoding nontypeable Haemophilus influenzae high-molecular-weight surface-exposed proteins related to filamentous hemagglutinin of Bordetella pertussis.
Barenkamp SJ, Leininger E
Infect. Immun. 1992 Apr;60(4):1302-13
PubMed ID: 1548058
The 100 kDa haem:haemopexin-binding protein of Haemophilus influenzae: structure and localization.
Cope LD, Thomas SE, Latimer JL, Slaughter CA, Müller-Eberhard U, Hansen EJ
Mol. Microbiol. 1994 Sep;13(5):863-73
PubMed ID: 7815944
Characterization of a novel two-partner secretion system implicated in the virulence of Pseudomonas aeruginosa.
Faure LM, Garvis S, de Bentzmann S, Bigot S
Microbiology (Reading, Engl.) 2014 Sep;160(Pt 9):1940-52
PubMed ID: 25009238