Pseudomonas aeruginosa PAO1, PA0767 (lepA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0767
Name
lepA
Replicon chromosome
Genomic location 835523 - 837322 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249458.1
GI 15595964
Affymetrix PA0767_lepA_at
Entrez 881998
GenBank AAG04156.1
INSDC AAG04156.1
NCBI Locus Tag PA0767
protein_id(GenBank) gb|AAG04156.1|AE004511_9|gnl|PseudoCAP|PA0767
TIGR NTL03PA00768
UniParc UPI000012E483
UniProtKB Acc Q9I5G8
UniProtKB ID LEPA_PSEAE
UniRef100 UniRef100_B7UYX5
UniRef50 UniRef50_O67618
UniRef90 UniRef90_A6VAK9

Product

Feature Type CDS
Coding Frame 1
Product Name
GTP-binding protein LepA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.08
Kyte-Doolittle Hydrophobicity Value -0.104
Molecular Weight (kDa) 66.3
Isoelectric Point (pI) 5.34

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000741 (550 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0767
Search term: lepA

Human Homologs

Ensembl 94, assembly GRCh38.p12
GUF1 homolog, GTPase [Source:HGNC Symbol;Acc:HGNC:25799]
E-value: 3.5e-170
Percent Identity: 48.4

References

GTP-binding membrane protein of Escherichia coli with sequence homology to initiation factor 2 and elongation factors Tu and G.
March PE, Inouye M
Proc. Natl. Acad. Sci. U.S.A. 1985 Nov;82(22):7500-4
PubMed ID: 2999765
Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8.
Kushiro M, Shimizu M, Tomita K
Eur. J. Biochem. 1987 Dec;170(1-2):93-8
PubMed ID: 2826164