Pseudomonas aeruginosa PAO1, PA0799

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA0799
Name
Replicon chromosome
Genomic location 876617 - 878608 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
CommentTranslation confirmed using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer. Coordinates verified using RT-PCR (PMID:24291602).

Cross-References

RefSeq NP_249490.1
GI 15595996
Affymetrix PA0799_at
Entrez 878028
GenBank AAG04188.1
INSDC AAG04188.1
NCBI Locus Tag PA0799
protein_id(GenBank) gb|AAG04188.1|AE004515_4|gnl|PseudoCAP|PA0799
TIGR NTL03PA00800
UniParc UPI00000C515C
UniProtKB Acc Q9I5D9
UniProtKB ID Q9I5D9_PSEAE
UniRef100 UniRef100_W0YMD9
UniRef50 UniRef50_A4XXC6
UniRef90 UniRef90_A6VAH6

Product

Feature Type CDS
Coding Frame 1
Product Name
probable helicase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.91
Kyte-Doolittle Hydrophobicity Value -0.270
Molecular Weight (kDa) 75.0
Isoelectric Point (pI) 6.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 397 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000772 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0799
Search term: probable helicase

Human Homologs

Ensembl 110, assembly GRCh38.p14
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 1 [Source:HGNC Symbol;Acc:HGNC:11097]
E-value: 1.5e-75
Percent Identity: 38.3

References

LTQ-XL mass spectrometry proteome analysis expands the Pseudomonas aeruginosa AmpR regulon to include cyclic di-GMP phosphodiesterases and phosphoproteins, and identifies novel open reading frames.
Kumari H, Murugapiran SK, Balasubramanian D, Schneper L, Merighi M, Sarracino D, Lory S, Mathee K
J Proteomics 2014 Jan 16;96:328-342
PubMed ID: 24291602