Pseudomonas aeruginosa PAO1, PA0843 (plcR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0843
Name
plcR
Replicon chromosome
Genomic location 918617 - 919240 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249534.1
GI 15596040
Affymetrix PA0843_plcR_at
DNASU PaCD00006387
Entrez 882099
GenBank AAG04232.1
NCBI Locus Tag PA0843
protein_id(GenBank) gb|AAG04232.1|AE004519_3|gnl|PseudoCAP|PA0843
TIGR NTL03PA00844
UniProtKB Acc P40695
UniProtKB ID PLCR_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
phospholipase accessory protein PlcR precursor
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7) -10.78
Kyte-Doolittle Hydrophobicity Value -0.520
Molecular Weight (kDa) 23.0
Isoelectric Point (pI) 4.44

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Extracellular Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 2 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000810 (302 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0843
Search term: plcR

References

A novel class of microbial phosphocholine-specific phospholipases C.
Stonehouse MJ, Cota-Gomez A, Parker SK, Martin WE, Hankin JA, Murphy RC, Chen W, Lim KB, Hackett M, Vasil AI, Vasil ML
Mol. Microbiol. 2002 Nov;46(3):661-76
PubMed ID: 12410824
Nucleotide sequences and expression in Escherichia coli of the in-phase overlapping Pseudomonas aeruginosa plcR genes.
Shen BF, Tai PC, Pritchard AE, Vasil ML
J. Bacteriol. 1987 Oct;169(10):4602-7
PubMed ID: 3115961
Mutations in the hemolytic-phospholipase C operon result in decreased virulence of Pseudomonas aeruginosa PAO1 grown under phosphate-limiting conditions.
Ostroff RM, Wretlind B, Vasil ML
Infect. Immun. 1989 May;57(5):1369-73
PubMed ID: 2496027
PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa.
Cota-Gomez A, Vasil AI, Kadurugamuwa J, Beveridge TJ, Schweizer HP, Vasil ML
Infect. Immun. 1997 Jul;65(7):2904-13
PubMed ID: 9199466