Pseudomonas aeruginosa PAO1, PA0867 (mliC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0867
Name
mliC
Replicon chromosome
Genomic location 948776 - 949159 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249558.1
GI 15596064
Affymetrix PA0867_at
DNASU PaCD00006398
Entrez 882238
GenBank AAG04256.1
INSDC AAG04256.1
NCBI Locus Tag PA0867
protein_id(GenBank) gb|AAG04256.1|AE004521_7|gnl|PseudoCAP|PA0867
TIGR NTL03PA00868
UniParc UPI00000C5199
UniProtKB Acc Q9I574
UniProtKB ID MLIC_PSEAE
UniRef100 UniRef100_Q9I574
UniRef50 UniRef50_Q9I574
UniRef90 UniRef90_Q9I574

Product

Feature Type CDS
Coding Frame 1
Product Name
membrane-bound lysozyme inhibitor of c-type lysozyme MliC
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected using LC-MS/MS (13.70 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.11
Kyte-Doolittle Hydrophobicity Value -0.224
Molecular Weight (kDa) 13.7
Isoelectric Point (pI) 4.83

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Vesicle Class 1
Outer Membrane Class 2
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3F6Z HYDROLASE 11/07/08 Crystal structure of Pseudomonas aeruginosa MliC in complex with hen egg white lysozyme PSEUDOMONAS AERUGINOSA; GALLUS GALLUS 2.5 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 33 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000834 (308 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0867
Search term: mliC

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
A new family of lysozyme inhibitors contributing to lysozyme tolerance in gram-negative bacteria.
Callewaert L, Aertsen A, Deckers D, Vanoirbeek KG, Vanderkelen L, Van Herreweghe JM, Masschalck B, Nakimbugwe D, Robben J, Michiels CW
PLoS Pathog. 2008 Mar;4(3):e1000019
PubMed ID: 18369469
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344