Pseudomonas aeruginosa PAO1, PA0972 (tolB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0972
Name
tolB
Replicon chromosome
Genomic location 1056049 - 1057347 (+ strand)

Cross-References

RefSeq NP_249663.1
GI 15596169
Affymetrix PA0972_tolB_at
Entrez 881864
GenBank AAG04361.1
INSDC AAC44661.2
NCBI Locus Tag PA0972
protein_id(GenBank) gb|AAG04361.1|AE004530_14|gnl|PseudoCAP|PA0972
TIGR NTL03PA00973
UniParc UPI000013710C
UniProtKB Acc P50601
UniProtKB ID TOLB_PSEAE
UniRef100 UniRef100_P50601
UniRef50 UniRef50_Q4K7E4
UniRef90 UniRef90_P50601

Product

Feature Type CDS
Coding Frame 1
Product Name
TolB protein
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (47.75 kDa, PMID:21751344)
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 3.48
Kyte-Doolittle Hydrophobicity Value -0.266
Molecular Weight (kDa) 47.7
Isoelectric Point (pI) 9.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 375 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000937 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0972
Search term: tolB
Search term: TolB protein

References

Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli.
Levengood SK, Webster RE
J. Bacteriol. 1989 Dec;171(12):6600-9
PubMed ID: 2687247
Maturation and localization of the TolB protein required for colicin import.
Isnard M, Rigal A, Lazzaroni JC, Lazdunski C, Lloubes R
J. Bacteriol. 1994 Oct;176(20):6392-6
PubMed ID: 7929011
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344