Pseudomonas aeruginosa PAO1, PA0997 (pqsB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA0997
Name	pqsB
Replicon	chromosome
Genomic location	1080009 - 1080860 (+ strand)
Transposon Mutants	3 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	Quinolone signal response

Cross-References

RefSeq	NP_249688.1
GI	15596194
Affymetrix	PA0997_at
DNASU	PaCD00006460
Entrez	883098
GenBank	AAG04386.1
INSDC	AAG04386.1
NCBI Locus Tag	PA0997
protein_id(GenBank)	gb|AAG04386.1|AE004532_10|gnl|PseudoCAP|PA0997
TIGR	NTL03PA00998
UniParc	UPI00000C51F9
UniProtKB Acc	Q9I4X2
UniProtKB ID	Q9I4X2_PSEAE
UniRef100	UniRef100_W0YKP3
UniRef50	UniRef50_Q02IG2
UniRef90	UniRef90_Q02IG2

Product

Feature Type	CDS
Coding Frame	1
Product Name	PqsB Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-11.88
Kyte-Doolittle Hydrophobicity Value	-0.050
Molecular Weight (kDa)	30.5
Isoelectric Point (pI)	4.60

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6ESZ	TRANSFERASE	10/25/17	Crystal structure of PqsBC from Pseudomonas aeruginosa (crystal form 1)	Pseudomonas aeruginosa PAO1	1.84	X-RAY DIFFRACTION	100.0
6ET0	TRANSFERASE	10/25/17	Crystal structure of PqsBC (C129A) mutant from Pseudomonas aeruginosa (crystal form 1)	Pseudomonas aeruginosa PAO1	1.53	X-RAY DIFFRACTION	100.0
6ET3	TRANSFERASE	10/25/17	Crystal structure of PqsBC (C129S) mutant from Pseudomonas aeruginosa (crystal form 4)	Pseudomonas aeruginosa PAO1	2.25	X-RAY DIFFRACTION	100.0
6ET1	TRANSFERASE	10/25/17	Crystal structure of PqsBC from Pseudomonas aeruginosa (crystal form 2)	Pseudomonas aeruginosa PAO1	2.65	X-RAY DIFFRACTION	100.0
6ET2	TRANSFERASE	10/25/17	Crystal structure of PqsBC (C129A) mutant from Pseudomonas aeruginosa (crystal form 3)	Pseudomonas aeruginosa PAO1	2.6	X-RAY DIFFRACTION	100.0
5DWZ	TRANSFERASE	09/23/15	Structural and functional characterization of PqsBC, a condensing enzyme in the biosynthesis of the Pseudomonas aeruginosa quinolone signal	Pseudomonas aeruginosa	2.04	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 13 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000960 (298 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA0997 Search term: pqsB Search term: PqsB

Human Homologs

References