Pseudomonas aeruginosa PAO1, PA1004 (nadA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1004
Name
nadA
Replicon chromosome
Genomic location 1087843 - 1088901 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentReaction not utilizing O2 is necessary to produce spermidine in anaerobic conditions, as shown by gap analysis and explained in references.

Cross-References

RefSeq NP_249695.1
GI 15596201
Affymetrix PA1004_nadA_at
Entrez 883095
GenBank AAG04393.1
INSDC AAG04393.1
NCBI Locus Tag PA1004
protein_id(GenBank) gb|AAG04393.1|AE004533_4|gnl|PseudoCAP|PA1004
TIGR NTL03PA01005
UniParc UPI000012FC67
UniProtKB Acc Q9I4W9
UniProtKB ID NADA_PSEAE
UniRef100 UniRef100_Q02IG9
UniRef50 UniRef50_Q8EEN5
UniRef90 UniRef90_Q02IG9

Product

Feature Type CDS
Coding Frame 1
Product Name
quinolinate synthetase A
Product Name Confidence: Class 2
Synonyms L-aspartate oxidase (aerobic/anaerobic) NadA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -9.71
Kyte-Doolittle Hydrophobicity Value -0.037
Molecular Weight (kDa) 38.4
Isoelectric Point (pI) 5.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 517 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000967 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1004
Search term: nadA

References

Molecular biology of pyridine nucleotide biosynthesis in Escherichia coli. Cloning and characterization of quinolinate synthesis genes nadA and nadB.
Flachmann R, Kunz N, Seifert J, Gütlich M, Wientjes FJ, Läufer A, Gassen HG
Eur. J. Biochem. 1988 Aug;175(2):221-8
PubMed ID: 2841129
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T
DNA Res. 1996 Jun;3(3):137-55
PubMed ID: 8905232
Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in nadB does not affect NAD biosynthesis or alginate production.
DeVries CA, Hassett DJ, Flynn JL, Ohman DE
Gene 1995 Apr;156(1):63-7
PubMed ID: 7737518