Pseudomonas aeruginosa PAO1, PA1014 (wapB)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1014
Name
wapB
Replicon chromosome
Genomic location 1097296 - 1098288 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 7 transposon mutants in orthologs
CommentResponsible for transfer of the terminal glucose residue to the outer core oligosaccharide in P. aeruginosa PAO1 LPS. In a knockout-mutant, the chemical structure of the LPS extracted from it lacked the terminal glucose residue as compared to the core oligosaccharide structure of the wild type strain PAO1.

Cross-References

RefSeq NP_249705.1
GI 15596211
Affymetrix PA1014_at
DNASU PaCD00006467
Entrez 883119
GenBank AAG04403.1
NCBI Locus Tag PA1014
protein_id(GenBank) gb|AAG04403.1|AE004534_1|gnl|PseudoCAP|PA1014
TIGR NTL03PA01015
UniProtKB Acc Q9I4V9
UniProtKB ID Q9I4V9_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
1,2-glucosyltransferase WapB
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.50
Kyte-Doolittle Hydrophobicity Value -0.272
Molecular Weight (kDa) 37.3
Isoelectric Point (pI) 8.33

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 551 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000682 (2168 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1014
Search term: wapB

References

Evidence that WapB is a 1,2-glucosyltransferase of Pseudomonas aeruginosa involved in Lipopolysaccharide outer core biosynthesis.
Kocíncová D, Hao Y, Vinogradov E, Lam JS
J. Bacteriol. 2011 Jun;193(11):2708-16
PubMed ID: 21441506