Pseudomonas aeruginosa PAO1, PA1070 (braG)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1070
Name
braG
Replicon chromosome
Genomic location 1157954 - 1158655 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249761.1
GI 15596267
Affymetrix PA1070_braG_at
DNASU PaCD00006492
Entrez 878069
GenBank AAG04459.1
INSDC AAG04459.1
NCBI Locus Tag PA1070
protein_id(GenBank) gb|AAG04459.1|AE004539_1|gnl|PseudoCAP|PA1070
TIGR NTL03PA01071
UniParc UPI0000126ABB
UniProtKB Acc P21630
UniProtKB ID BRAG_PSEAE
UniRef100 UniRef100_P21630
UniRef50 UniRef50_P22731
UniRef90 UniRef90_P21630

Product

Feature Type CDS
Coding Frame 1
Product Name
branched-chain amino acid transport protein BraG
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.55
Kyte-Doolittle Hydrophobicity Value -0.044
Molecular Weight (kDa) 25.6
Isoelectric Point (pI) 6.55

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 669 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001032 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1070
Search term: braG

Human Homologs

Ensembl 94, assembly GRCh38.p12
ATP binding cassette subfamily B member 5 [Source:HGNC Symbol;Acc:HGNC:46]
E-value: 3.9e-16
Percent Identity: 30.2

References

Cloning, nucleotide sequences, and identification of products of the Pseudomonas aeruginosa PAO bra genes, which encode the high-affinity branched-chain amino acid transport system.
Hoshino T, Kose K
J. Bacteriol. 1990 Oct;172(10):5531-9
PubMed ID: 2120183