Pseudomonas aeruginosa PAO1, PA1097 (fleQ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1097
Name
fleQ
Replicon chromosome
Genomic location 1187587 - 1189059 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249788.1
GI 15596294
Affymetrix PA1097_fleQ_at
Entrez 881960
GenBank AAG04486.1
INSDC AAG04486.1
NCBI Locus Tag PA1097
protein_id(GenBank) gb|AAG04486.1|AE004540_6|gnl|PseudoCAP|PA1097
TIGR NTL03PA01098
UniParc UPI00000D421B
UniProtKB Acc G3XCV0
UniProtKB ID G3XCV0_PSEAE
UniRef100 UniRef100_G3XCV0
UniRef50 UniRef50_G3XCV0
UniRef90 UniRef90_G3XCV0

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator FleQ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.91
Kyte-Doolittle Hydrophobicity Value -0.364
Molecular Weight (kDa) 55.3
Isoelectric Point (pI) 5.03

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
4WXM TRANSCRIPTION REGULATOR 11/14/14 FleQ REC domain from Pseudomonas aeruginosa PAO1 PSEUDOMONAS AERUGINOSA PAO1 2.3 X-RAY DIFFRACTION 100.0
5EXX TRANSCRIPTION 11/24/15 AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to c-di-GMP PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 3.311 X-RAY DIFFRACTION 100.0
5EXS TRANSCRIPTION 11/24/15 AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to ATP-gamma-S PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 100.0
5EXT TRANSCRIPTION 11/24/15 AAA+ domain of FleQ from Pseudomonas aeruginosa bound to ADP PSEUDOMONAS AERUGINOSA 2.4 X-RAY DIFFRACTION 100.0
5EXP TRANSCRIPTION 11/24/15 AAA+ domain of FleQ from Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 453 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001058 (549 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1097
Search term: fleQ

References

FleQ, the major flagellar gene regulator in Pseudomonas aeruginosa, binds to enhancer sites located either upstream or atypically downstream of the RpoN binding site.
Jyot J, Dasgupta N, Ramphal R
J. Bacteriol. 2002 Oct;184(19):5251-60
PubMed ID: 12218010
A transcriptional activator, FleQ, regulates mucin adhesion and flagellar gene expression in Pseudomonas aeruginosa in a cascade manner.
Arora SK, Ritchings BW, Almira EC, Lory S, Ramphal R
J. Bacteriol. 1997 Sep;179(17):5574-81
PubMed ID: 9287015