Pseudomonas aeruginosa PAO1, PA1174 (napA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1174
Name
napA
Replicon chromosome
Genomic location 1273309 - 1275798 (- strand)
Transposon Mutants 11 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249865.1
GI 15596371
Affymetrix PA1174_napA_at
Entrez 878409
GenBank AAG04563.1
INSDC AAG04563.1
NCBI Locus Tag PA1174
protein_id(GenBank) gb|AAG04563.1|AE004547_9|gnl|PseudoCAP|PA1174
TIGR NTL03PA01175
UniParc UPI00000C528C
UniProtKB Acc Q9I4G3
UniProtKB ID NAPA_PSEAE
UniRef100 UniRef100_Q9I4G3
UniRef50 UniRef50_Q53176
UniRef90 UniRef90_A6V924

Product

Feature Type CDS
Coding Frame 1
Product Name
periplasmic nitrate reductase protein NapA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 10.37
Kyte-Doolittle Hydrophobicity Value -0.359
Molecular Weight (kDa) 92.9
Isoelectric Point (pI) 8.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 487 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001132 (349 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1174
Search term: napA

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
Structure and function of a periplasmic nitrate reductase in Alcaligenes eutrophus H16.
Siddiqui RA, Warnecke-Eberz U, Hengsberger A, Schneider B, Kostka S, Friedrich B
J. Bacteriol. 1993 Sep;175(18):5867-76
PubMed ID: 8376334