Pseudomonas aeruginosa PAO1, PA1342 (aatJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1342
Name
aatJ
Replicon chromosome
Genomic location 1455815 - 1456723 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250033.1
GI 15596539
Affymetrix PA1342_at
DNASU PaCD00006620
Entrez 879321
GenBank AAG04731.1
INSDC AAG04731.1
NCBI Locus Tag PA1342
protein_id(GenBank) gb|AAG04731.1|AE004563_8|gnl|PseudoCAP|PA1342
TIGR NTL03PA01343
UniParc UPI00000C5325
UniProtKB Acc Q9I402
UniProtKB ID Q9I402_PSEAE
UniRef100 UniRef100_W0YPF9
UniRef50 UniRef50_P37902
UniRef90 UniRef90_W0YPF9

Product

Feature Type CDS
Coding Frame 1
Product Name
AatJ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected by iTRAQ (PMID:21863142).
Charge (pH 7) 2.66
Kyte-Doolittle Hydrophobicity Value -0.246
Molecular Weight (kDa) 33.1
Isoelectric Point (pI) 8.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 2
Periplasmic Class 3
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 372 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001277 (512 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1342
Search term: aatJ
Search term: AatJ

References

Cloning and characterization of a glutamine transport operon of Bacillus stearothermophilus NUB36: effect of temperature on regulation of transcription.
Wu L, Welker NE
J. Bacteriol. 1991 Aug;173(15):4877-88
PubMed ID: 1856180
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142
Characterization of a Pseudomonas putida ABC transporter (AatJMQP) required for acidic amino acid uptake: biochemical properties and regulation by the Aau two-component system.
Singh B, Röhm KH
Microbiology (Reading, Engl.) 2008 Mar;154(Pt 3):797-809
PubMed ID: 18310026
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J. Bacteriol. 2015 Feb;197(4):762-73
PubMed ID: 25488299