Pseudomonas aeruginosa PAO1, PA1342 (aatJ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA1342
Name
aatJ
Replicon chromosome
Genomic location 1455815 - 1456723 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250033.1
GI 15596539
Affymetrix PA1342_at
DNASU PaCD00006620
Entrez 879321
GenBank AAG04731.1
INSDC AAG04731.1
NCBI Locus Tag PA1342
protein_id(GenBank) gb|AAG04731.1|AE004563_8|gnl|PseudoCAP|PA1342
TIGR NTL03PA01343
UniParc UPI00000C5325
UniProtKB Acc Q9I402
UniProtKB ID Q9I402_PSEAE
UniRef100 UniRef100_W0YPF9
UniRef50 UniRef50_P37902
UniRef90 UniRef90_W0YPF9

Product

Feature Type CDS
Coding Frame 1
Product Name
AatJ
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by iTRAQ (PMID:21863142).
Charge (pH 7) 2.66
Kyte-Doolittle Hydrophobicity Value -0.246
Molecular Weight (kDa) 33.1
Isoelectric Point (pI) 8.60

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Extracellular Class 1
mass spectrometry evidence used in manual assertion
Periplasmic Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 372 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001277 (512 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1342
Search term: aatJ
Search term: AatJ

References

Cloning and characterization of a glutamine transport operon of Bacillus stearothermophilus NUB36: effect of temperature on regulation of transcription.
Wu L, Welker NE
J Bacteriol 1991 Aug;173(15):4877-88
PubMed ID: 1856180
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011 ;2
PubMed ID: 21863142
Characterization of a Pseudomonas putida ABC transporter (AatJMQP) required for acidic amino acid uptake: biochemical properties and regulation by the Aau two-component system.
Singh B, Röhm KH
Microbiology (Reading, Engl.) 2008 Mar;154(Pt 3):797-809
PubMed ID: 18310026
Mep72, a metzincin protease that is preferentially secreted by biofilms of Pseudomonas aeruginosa.
Passmore IJ, Nishikawa K, Lilley KS, Bowden SD, Chung JC, Welch M
J. Bacteriol. 2015 Feb;197(4):762-73
PubMed ID: 25488299