Pseudomonas aeruginosa PAO1, PA1400

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1400
Name
Replicon chromosome
Genomic location 1521109 - 1524396 (+ strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250091.1
GI 15596597
Affymetrix PA1400_at
Entrez 879389
GenBank AAG04789.1
INSDC AAG04789.1
NCBI Locus Tag PA1400
protein_id(GenBank) gb|AAG04789.1|AE004569_7|gnl|PseudoCAP|PA1400
TIGR NTL03PA01401
UniParc UPI00000C535D
UniProtKB Acc Q9I3U4
UniProtKB ID Q9I3U4_PSEAE
UniRef100 UniRef100_Q9I3U4
UniRef50 UniRef50_Q1LDE3
UniRef90 UniRef90_Q9I3U4

Product

Feature Type CDS
Coding Frame 1
Product Name
probable pyruvate carboxylase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -21.33
Kyte-Doolittle Hydrophobicity Value 0.033
Molecular Weight (kDa) 116.8
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 629 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001321 (325 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1400

Human Homologs

Ensembl 94, assembly GRCh38.p12
pyruvate carboxylase [Source:HGNC Symbol;Acc:HGNC:8636]
E-value: 5.8e-102
Percent Identity: 42.8

References

Pyruvate carboxylase deficiency in pleiotropic carbohydrate-negative mutant strains of Pseudomonas aeruginosa.
Phibbs PV, Feary TW, Blevins WT
J. Bacteriol. 1974 Jun;118(3):999-1009
PubMed ID: 4208415
Some properties of the pyruvate carboxylase from Pseudomonas fluorescens.
Milrad de Forchetti SR, Cazzulo JJ
J. Gen. Microbiol. 1976 Mar;93(1):75-81
PubMed ID: 4579
Clustering of mutations affecting central pathway enzymes of carbohydrate catabolism in Pseudomonas aeruginosa.
Roehl RA, Feary TW, Phibbs PV
J. Bacteriol. 1983 Dec;156(3):1123-9
PubMed ID: 6417110