Pseudomonas aeruginosa PAO1, PA1421 (gbuA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1421
Name
gbuA
Synonym: speB2
Replicon chromosome
Genomic location 1546271 - 1547230 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250112.1
GI 15596618
Affymetrix PA1421_speB2_at
DNASU PaCD00006654
Entrez 881747
GenBank AAG04810.1
INSDC AAG04810.1
NCBI Locus Tag PA1421
protein_id(GenBank) gb|AAG04810.1|AE004571_9|gnl|PseudoCAP|PA1421
TIGR NTL03PA01422
UniParc UPI00000C5372
UniProtKB Acc Q9I3S3
UniProtKB ID GBUA_PSEAE
UniRef100 UniRef100_Q9I3S3
UniRef50 UniRef50_Q9I3S3
UniRef90 UniRef90_Q9I3S3

Product

Feature Type CDS
Coding Frame 1
Product Name
guanidinobutyrase
Product Name Confidence: Class 1
Synonyms 4-guanidinobutanoate amidinohydrolase
agmatinase, agmatine ureohydrolase, AUH, proclavaminate amidino hydrolase, proclavaminic acid amidino hydrolase, PAH
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.74
Kyte-Doolittle Hydrophobicity Value -0.039
Molecular Weight (kDa) 34.7
Isoelectric Point (pI) 5.07

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3NIO HYDROLASE 06/16/10 Crystal structure of Pseudomonas aeruginosa guanidinobutyrase PSEUDOMONAS AERUGINOSA 2.0 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 434 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001341 (492 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1421
Search term: gbuA
Search term: guanidinobutyrase

Human Homologs

Ensembl 94, assembly GRCh38.p12
agmatinase [Source:HGNC Symbol;Acc:HGNC:18407]
E-value: 1.0e-112
Percent Identity: 61.6

References

Purification and properties of agmatine ureohydrolyase, a putrescine biosynthetic enzyme in Escherichia coli.
Satishchandran C, Boyle SM
J. Bacteriol. 1986 Mar;165(3):843-8
PubMed ID: 3081491
Cloning, sequencing and disruption of a gene from Streptomyces clavuligerus involved in clavulanic acid biosynthesis.
Aidoo KA, Wong A, Alexander DC, Rittammer RA, Jensen SE
Gene 1994 Sep;147(1):41-6
PubMed ID: 8088547
Identification, cloning, sequencing, and overexpression of the gene encoding proclavaminate amidino hydrolase and characterization of protein function in clavulanic acid biosynthesis.
Wu TK, Busby RW, Houston TA, McIlwaine DB, Egan LA, Townsend CA
J. Bacteriol. 1995 Jul;177(13):3714-20
PubMed ID: 7601835