Pseudomonas aeruginosa PAO1, PA1430 (lasR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA1430
Name
lasR
Replicon chromosome
Genomic location 1558171 - 1558890 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_250121.1
GI 15596627
Affymetrix PA1430_lasR_at
DNASU PaCD00006658
Entrez 881789
GenBank AAG04819.1
INSDC AAG04819.1
NCBI Locus Tag PA1430
protein_id(GenBank) gb|AAG04819.1|AE004572_7|gnl|PseudoCAP|PA1430
TIGR NTL03PA01431
UniParc UPI000012E231
UniProtKB Acc P25084
UniProtKB ID LASR_PSEAE
UniRef100 UniRef100_P25084
UniRef50 UniRef50_P25084
UniRef90 UniRef90_P25084

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator LasR
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.68
Kyte-Doolittle Hydrophobicity Value -0.020
Molecular Weight (kDa) 26.6
Isoelectric Point (pI) 6.51

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Cytoplasmic Class 1
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2UV0 TRANSCRIPTION 03/08/07 Structure of the P. aeruginosa LasR ligand-binding domain bound to its autoinducer PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
3IX3 TRANSCRIPTION 09/03/09 LasR-OC12 HSL complex PSEUDOMONAS AERUGINOSA 1.4 X-RAY DIFFRACTION 100.0
3IX8 TRANSCRIPTION 09/03/09 LasR-TP3 complex PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
6D6C signaling protein/agonist 04/20/18 The structure of ligand binding domain of LasR in complex with TP-1 homolog, compound 12 PSEUDOMONAS AERUGINOSA PAO1 1.88 X-RAY DIFFRACTION 100.0
3IX4 TRANSCRIPTION 09/03/09 LasR-TP1 complex PSEUDOMONAS AERUGINOSA 1.8 X-RAY DIFFRACTION 100.0
3JPU TRANSCRIPTION 09/04/09 LasR-TP4 complex PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-3-OXO-DODECANOYL-L-HOMOSERINE LACTONE DB08324 DrugBank 5.1.1 P25084 Transcriptional activator protein LasR 100.0 239 1.7e-135

Virulence Evidence

Source 1
Database Victors : 4258
Category N-(3-oxo-dodecanoyl)-L-homoserine lactone QS system
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 8557368
Source 2
Database VFDB : VF0093
Category N-(3-oxo-dodecanoyl)-L-homoserine lactone QS system
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 142 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001096 (1475 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1430
Search term: lasR

References

Purification and characterization of LasR as a DNA-binding protein.
You Z, Fukushima J, Ishiwata T, Chang B, Kurata M, Kawamoto S, Williams P, Okuda K
FEMS Microbiol. Lett. 1996 Sep;142(2-3):301-7
PubMed ID: 8810514
Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression.
Gambello MJ, Iglewski BH
J. Bacteriol. 1991 May;173(9):3000-9
PubMed ID: 1902216
Intracellular receptor-type transcription factor, LasR, contains a highly conserved amphipathic region which precedes the putative helix-turn-helix DNA binding motif.
Fukushima J, Ishiwata T, Kurata M, You Z, Okuda K
Nucleic Acids Res. 1994 Sep;22(18):3706-7
PubMed ID: 7937080