Pseudomonas aeruginosa PAO1, PA1432 (lasI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1432
Name
lasI
Replicon chromosome
Genomic location 1559254 - 1559859 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_250123.1
GI 15596629
Affymetrix PA1432_lasI_at
DNASU PaCD00006659
Entrez 881777
GenBank AAG04821.1
INSDC AAG04821.1
NCBI Locus Tag PA1432
protein_id(GenBank) gb|AAG04821.1|AE004572_9|gnl|PseudoCAP|PA1432
TIGR NTL03PA01433
UniParc UPI000012E230
UniProtKB Acc P33883
UniProtKB ID LASI_PSEAE
UniRef100 UniRef100_P33883
UniRef50 UniRef50_P33883
UniRef90 UniRef90_P33883

Product

Feature Type CDS
Coding Frame 1
Product Name
autoinducer synthesis protein LasI
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.13
Kyte-Doolittle Hydrophobicity Value -0.104
Molecular Weight (kDa) 22.7
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1RO5 SIGNALING PROTEIN 12/01/03 Crystal Structure of the AHL Synthase LasI Pseudomonas aeruginosa 2.3 X-RAY DIFFRACTION 98.0

Virulence Evidence

Source 1
Database VFDB : VF0093
Category N-(3-oxo-dodecanoyl)-L-homoserine lactone QS system
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 58 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001349 (304 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1432
Search term: lasI

Human Homologs

References

Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication.
Passador L, Cook JM, Gambello MJ, Rust L, Iglewski BH
Science 1993 May 21;260(5111):1127-30
PubMed ID: 8493556
Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.
Pearson JP, Gray KM, Passador L, Tucker KD, Eberhard A, Iglewski BH, Greenberg EP
Proc Natl Acad Sci U S A 1994 Jan 4;91(1):197-201
PubMed ID: 8278364