Pseudomonas aeruginosa UCBPP-PA14, PA14_05530 (mexA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_05530
Name
mexA
Replicon chromosome
Genomic location 486775 - 487926 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_788599.1
GI 116054156
Entrez 4384293
INSDC ABJ15392.1
NCBI Locus Tag PA14_05530
UniParc UPI000012F021
UniProtKB Acc Q02TY7
UniProtKB ID Q02TY7_PSEAB
UniRef100 UniRef100_P52477
UniRef50 UniRef50_P52477
UniRef90 UniRef90_P52477

Product

Feature Type CDS
Coding Frame 1
Product Name
RND multidrug efflux membrane fusion protein MexA
Synonyms
Evidence for Translation
Charge (pH 7) 3.22
Kyte-Doolittle Hydrophobicity Value -0.229
Molecular Weight (kDa) 41.0
Isoelectric Point (pI) 9.15

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexA MexA is the membrane fusion protein of the MexAB-OprM multidrug efflux complex. ARO:3000377 protein homolog model Perfect 716.1 100.0 3.0.1

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2V4D MEMBRANE PROTEIN 09/18/08 Re-refinement of MexA adaptor protein PSEUDOMONAS AERUGINOSA 3.2 X-RAY DIFFRACTION 100.0
1T5E TRANSPORT PROTEIN 05/04/04 The structure of MexA PSEUDOMONAS AERUGINOSA 3.0 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
Glycerin DB09462 DrugBank 5.1.1 P52477 Multidrug resistance protein MexA 100.0 383 2.1e-204

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration model
Reference 9989496
Source 2
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Caenorhabditis elegans
Notes
Infection model C. elegans fast killing model
Reference 9989496

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 394 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000416 (543 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_05530
Search term: mexA

References

No references are associated with this feature.