Pseudomonas aeruginosa UCBPP-PA14, PA14_08370 (vfr)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_08370
Name	vfr
Replicon	chromosome
Genomic location	717012 - 717656 (- strand)
Transposon Mutants	1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	5 transposon mutants in orthologs

Cross-References

RefSeq	YP_788822.1
GI	116054377
Entrez	4383977
INSDC	ABJ15613.1
NCBI Locus Tag	PA14_08370
UniParc	UPI0000138482
UniProtKB Acc	Q02TB4
UniProtKB ID	Q02TB4_PSEAB
UniRef100	UniRef100_P55222
UniRef50	UniRef50_P29281
UniRef90	UniRef90_P55222

Product

Feature Type	CDS
Coding Frame	1
Product Name	cAMP-regulatory protein
Synonyms
Evidence for Translation
Charge (pH 7)	3.29
Kyte-Doolittle Hydrophobicity Value	-0.250
Molecular Weight (kDa)	24.2
Isoelectric Point (pI)	8.22

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7FF0	DNA BINDING PROTEIN	07/22/21	Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and auranofin	Pseudomonas aeruginosa PAO1	2.6	X-RAY DIFFRACTION	100.0
7FF9	DNA BINDING PROTEIN	07/23/21	Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)	Pseudomonas aeruginosa PAO1	2.4	X-RAY DIFFRACTION	100.0
7FEW	DNA BINDING PROTEIN	07/22/21	Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and auranofin gold analogues	Pseudomonas aeruginosa PAO1	1.75	X-RAY DIFFRACTION	100.0
2OZ6	DNA BINDING PROTEIN	02/24/07	Crystal Structure of Virulence Factor Regulator from Pseudomonas aeruginosa in complex with cAMP	Pseudomonas aeruginosa	2.8	X-RAY DIFFRACTION	100.0
7FF8	DNA BINDING PROTEIN	07/22/21	Pseudomonas aeruginosa Virulence Factor Regulator with cAMP ligand and Cl(triethylphosphine)gold(I)	Pseudomonas aeruginosa PAO1	2.8	X-RAY DIFFRACTION	99.5

Virulence Evidence

Source 1
Database	PseudoCAP
Category
Evidence	ECO:0000015 mutant phenotype evidence
Host Organism	Caenorhabditis elegans
Notes
Infection model	C. elegans slow killing assay.
Reference	22911607

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 443 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG000272 (963 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_08370 Search term: vfr Search term: cAMP-regulatory protein

Human Homologs

References