Pseudomonas aeruginosa UCBPP-PA14, PA14_10790 (ampC)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_10790
Name	ampC
Replicon	chromosome
Genomic location	932631 - 933824 (- strand)
Transposon Mutants	1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	3 transposon mutants in orthologs

Cross-References

RefSeq	YP_789012.1
GI	116052144
EC Number	3.5.2.6
Entrez	4382097
INSDC	ABJ13380.1
NCBI Locus Tag	PA14_10790
UniParc	UPI000039629D
UniProtKB Acc	Q02SS4
UniProtKB ID	Q02SS4_PSEAB
UniRef100	UniRef100_Q02SS4
UniRef50	UniRef50_P85302
UniRef90	UniRef90_P24735

Product

Feature Type	CDS
Coding Frame	1
Product Name	beta-lactamase
Synonyms
Evidence for Translation
Charge (pH 7)	4.38
Kyte-Doolittle Hydrophobicity Value	-0.296
Molecular Weight (kDa)	43.5
Isoelectric Point (pI)	8.82

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name	Definition	Accession	Model Type (?)	SNP	Cutoff	Bit Score	Percent Identity	CARD Version
PDC-34	PDC-34 is a PDC beta-lactamase. []	ARO:3006709	protein homolog model		Perfect	806.6	100.0	3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
8SDS	HYDROLASE	04/07/23	Crystal structure of PDC-3 Y221H beta-lactamase in complex with the boronic acid inhibitor LP-06	Pseudomonas aeruginosa	1.63	X-RAY DIFFRACTION	98.5
6UQT	ANTIBIOTIC	10/21/19	Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors	Pseudomonas aeruginosa	1.25	X-RAY DIFFRACTION	98.6
6S1S	HYDROLASE	06/19/19	Crystal structure of AmpC from Pseudomonas aeruginosa in complex with [3-(2-carboxyvinyl)phenyl]boronic acid] inhibitor	Pseudomonas aeruginosa	1.78	X-RAY DIFFRACTION	98.5
8SDN	HYDROLASE	04/07/23	Crystal structure of PDC-3 Y221H beta-lactamase	Pseudomonas aeruginosa	2.1	X-RAY DIFFRACTION	98.5
4WZ4	hydrolase/hydrolase inhibitor	11/18/14	Crystal structure of P. aeruginosa AmpC	Pseudomonas aeruginosa PAO1	1.05	X-RAY DIFFRACTION	98.9
4X68	HYDROLASE/HYDROLASE INHIBITOR	12/07/14	Crystal Structure of OP0595 complexed with AmpC	Pseudomonas aeruginosa	1.68	X-RAY DIFFRACTION	98.9
4GZB	HYDROLASE	09/06/12	Crystal structure of native AmpC beta-lactamase from Pseudomonas aeruginosa PAO1	Pseudomonas aeruginosa	1.79	X-RAY DIFFRACTION	98.6
3S1Y	Hydrolase/Hydrolase Inhibitor	05/16/11	AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA) in complex with a beta-lactamase inhibitor	Pseudomonas aeruginosa	1.4	X-RAY DIFFRACTION	98.6
4OOY	hydrolase/hydrolase inhibitor	02/04/14	Avibactam and class C beta-lactamases: mechanism of inhibition, conservation of binding pocket and implications for resistance	Pseudomonas aeruginosa	1.1	X-RAY DIFFRACTION	98.9
6UQS	ANTIBIOTIC	10/21/19	Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors	Pseudomonas aeruginosa	1.37	X-RAY DIFFRACTION	98.6
8SDR	HYDROLASE/HYDROLASE INHIBITOR	04/07/23	Crystal structure of PDC-3 beta-lactamase in complex with the boronic acid inhibitor LP-06	Pseudomonas aeruginosa	1.35	X-RAY DIFFRACTION	98.7
6I30	HYDROLASE	11/02/18	Crystal structure of the AmpC from Pseudomonas aeruginosa with 1C	Pseudomonas aeruginosa	2.21	X-RAY DIFFRACTION	98.9
4NK3	HYDROLASE/HYDROLASE INHIBITOR	11/12/13	Amp-c beta-lactamase (pseudomonas aeruginosa) in complex with mk-7655	Pseudomonas aeruginosa	1.9	X-RAY DIFFRACTION	98.6
8SDL	HYDROLASE	04/07/23	Crystal structure of PDC-3 beta-lactamase	Pseudomonas aeruginosa	1.75	X-RAY DIFFRACTION	98.7
3S22	Hydrolase/Hydrolase Inhibitor	05/16/11	AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA) in complex with an inhibitor	Pseudomonas aeruginosa	1.65	X-RAY DIFFRACTION	98.6
4HEF	HYDROLASE/HYDROLASE INHIBITOR	10/03/12	Structure of avibactam bound to Pseudomonas aeruginosa AmpC	Pseudomonas aeruginosa	1.86	X-RAY DIFFRACTION	98.6
2WZX	HYDROLASE	12/03/09	AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA)IN COMPLEX WITH compound M-02	PSEUDOMONAS AERUGINOSA	1.4	X-RAY DIFFRACTION	98.6
8SDT	HYDROLASE	04/07/23	Crystal structure of PDC-3 beta-lactamase in complex with the boronic acid inhibitor S02030	Pseudomonas aeruginosa	1.38	X-RAY DIFFRACTION	98.7
2WZZ	HYDROLASE	12/03/09	AMP-C BETA-LACTAMASE (PSEUDOMONAS AERUGINOSA)IN COMPLEX WITH compound M-03	PSEUDOMONAS AERUGINOSA	1.57	X-RAY DIFFRACTION	98.6
6UR3	ANTIBIOTIC	10/22/19	Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors	Pseudomonas aeruginosa	1.423	X-RAY DIFFRACTION	98.6
4WYY	hydrolase/hydrolase inhibitor	11/18/14	Crystal Structure of P. aeruginosa AmpC	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)	1.28	X-RAY DIFFRACTION	98.9
6UQU	ANTIBIOTIC	10/21/19	Serendipitous Discovery of Aryl Boronic Acids as beta-Lactamase Inhibitors	Pseudomonas aeruginosa	1.0902	X-RAY DIFFRACTION	98.6
8SDV	HYDROLASE	04/07/23	Crystal structure of PDC-3 Y221H beta-lactamase in complex with the boronic acid inhibitor S02030	Pseudomonas aeruginosa	1.42	X-RAY DIFFRACTION	98.5

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 275 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001588 (493 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_10790 Search term: ampC Search term: beta-lactamase

Human Homologs

References