Pseudomonas aeruginosa UCBPP-PA14, PA14_15990 (trmD)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_15990
Name	trmD
Replicon	chromosome
Genomic location	1361846 - 1362604 (+ strand)
Transposon Mutants	1 transposon mutants in UCBPP-PA14

Cross-References

RefSeq	YP_789420.1
GI	116051741
EC Number	2.1.1.31
Entrez	4381629
INSDC	ABJ12977.1
NCBI Locus Tag	PA14_15990
UniParc	UPI000039617A
UniProtKB Acc	Q02RL6
UniProtKB ID	TRMD_PSEAB
UniRef100	UniRef100_Q02RL6
UniRef50	UniRef50_B2JF31
UniRef90	UniRef90_A6V125

Product

Feature Type	CDS
Coding Frame	1
Product Name	tRNA (guanine-N(1)-)-methyltransferase
Synonyms
Evidence for Translation
Charge (pH 7)	-8.61
Kyte-Doolittle Hydrophobicity Value	-0.430
Molecular Weight (kDa)	28.3
Isoelectric Point (pI)	4.98

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6AFK	TRANSFERASE	08/08/18	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa UCBPP-PA14	2.75	X-RAY DIFFRACTION	100.0
5WYR	TRANSFERASE	01/15/17	Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain UCBPP-PA14)	2.45	X-RAY DIFFRACTION	100.0
5WYQ	TRANSFERASE	01/15/17	Crystal Structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa	Pseudomonas aeruginosa (strain UCBPP-PA14)	2.16	X-RAY DIFFRACTION	100.0
5ZHN	TRANSFERASE	03/13/18	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa UCBPP-PA14	2.65	X-RAY DIFFRACTION	100.0
6JOE	TRANSFERASE	03/20/19	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa UCBPP-PA14	2.21	X-RAY DIFFRACTION	100.0
5ZHM	TRANSFERASE	03/13/18	Crystal structure of TrmD from Pseudomonas aeruginosa in complex with active-site inhibitor	Pseudomonas aeruginosa	2.76	X-RAY DIFFRACTION	100.0
6JKI	TRANSFERASE	03/01/19	Crystal structure and catalytic mechanism of the essential m1G37 tRNA methyltransferase TrmD from Pseudomonas aeruginosa	Pseudomonas aeruginosa	2.59	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001939 (537 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_15990 Search term: trmD Search term: tRNA (guanine-N(1)-)-methyltransferase

Human Homologs

References