Pseudomonas aeruginosa UCBPP-PA14, PA14_16250 (lasB)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_16250
Name	lasB
Replicon	chromosome
Genomic location	1387521 - 1389017 (+ strand)
Transposon Mutants	2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	4 transposon mutants in orthologs

Cross-References

RefSeq	YP_789440.1
GI	116051721
Entrez	4380221
INSDC	ABJ12957.1
NCBI Locus Tag	PA14_16250
UniParc	UPI000059F56A
UniProtKB Acc	Q02RJ6
UniProtKB ID	ELAS_PSEAB
UniRef100	UniRef100_Q02RJ6
UniRef50	UniRef50_P14756
UniRef90	UniRef90_P14756

Product

Feature Type	CDS
Coding Frame	1
Product Name	elastase LasB
Synonyms
Evidence for Translation
Charge (pH 7)	-1.48
Kyte-Doolittle Hydrophobicity Value	-0.264
Molecular Weight (kDa)	53.6
Isoelectric Point (pI)	6.74

Subcellular localization

Individual Mappings	Localization Confidence PMID Extracellular Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
7AJR	HYDROLASE	09/29/20	Virtual screening approach leading to the identification of a novel and tractable series of Pseudomonas aeruginosa elastase inhibitors	Pseudomonas aeruginosa	1.75	X-RAY DIFFRACTION	98.7
7OC7	HYDROLASE	04/26/21	LasB, alpha-alkyl-N-aryl mercaptoacetamide	Pseudomonas aeruginosa	1.95	X-RAY DIFFRACTION	100.0
6F8B	HYDROLASE	12/12/17	LasB bound to thiol based inhibitor	Pseudomonas aeruginosa	1.3	X-RAY DIFFRACTION	98.7
7NLM	HYDROLASE	02/22/21	LasB, N-aryl-2-butylmercaptoacetamide	Pseudomonas aeruginosa (strain UCBPP-PA14)	1.65	X-RAY DIFFRACTION	100.0
1EZM	HYDROLASE	01/13/92	THREE-DIMENSIONAL STRUCTURE OF THE ELASTASE OF PSEUDOMONAS AERUGINOSA AT 1.5 ANGSTROMS RESOLUTION	Pseudomonas aeruginosa	1.5	X-RAY DIFFRACTION	98.7
7NLK	HYDROLASE	02/22/21	LasB, N-aryl-2-butylmercaptoacetamide	Pseudomonas aeruginosa (strain UCBPP-PA14)	1.7	X-RAY DIFFRACTION	100.0
6FZX	HYDROLASE	03/15/18	LasB, hydroxymate Inhibitor Complex	Pseudomonas aeruginosa	2.1	X-RAY DIFFRACTION	98.7
8CR7	HYDROLASE	03/07/23	Crystal structure of recombinant LasB from Pseudomonas aeruginosa PA7	Pseudomonas aeruginosa PA7	1.5	X-RAY DIFFRACTION	94.9
1U4G	HYDROLASE	07/25/04	Elastase of Pseudomonas aeruginosa with an inhibitor	Pseudomonas aeruginosa	1.4	X-RAY DIFFRACTION	98.7
7QH1	HYDROLASE	12/10/21	Discovery and development of a novel inhaled antivirulence therapy for the treatment of Pseudomonas aeruginosa infections in patients with chronic respiratory disease	Pseudomonas aeruginosa	2.74	X-RAY DIFFRACTION	98.7
8CR4	HYDROLASE	03/07/23	Crystal structure of recombinant LasBArtif from Pseudomonas aeruginosa AZPAE14816	Pseudomonas aeruginosa	0.91	X-RAY DIFFRACTION	95.5
4K89	HYDROLASE	04/18/13	Crystal structure of Pseudomonas aeruginosa strain K solvent tolerant elastase	Pseudomonas aeruginosa	1.39	X-RAY DIFFRACTION	99.0
3DBK	HYDROLASE	06/01/08	Pseudomonas aeruginosa elastase with phosphoramidon	Pseudomonas aeruginosa	1.4	X-RAY DIFFRACTION	98.7
8CR3	HYDROLASE	03/07/23	Crystal structure of recombinant LasB from Pseudomonas aeruginosa PAO1	Pseudomonas aeruginosa PAO1	1.12	X-RAY DIFFRACTION	97.3

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 114 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001959 (310 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_16250 Search term: lasB Search term: elastase LasB

Human Homologs

References