Pseudomonas aeruginosa UCBPP-PA14, PA14_49560 (toxA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_49560
Name
toxA
Replicon chromosome
Genomic location 4407441 - 4409348 (+ strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_792118.1
GI 116049080
Entrez 4381678
INSDC ABJ10316.1
NCBI Locus Tag PA14_49560
UniParc UPI000045D0C3
UniProtKB Acc Q02IW8
UniProtKB ID Q02IW8_PSEAB
UniRef100 UniRef100_Q02IW8
UniRef50 UniRef50_P11439
UniRef90 UniRef90_P11439

Product

Feature Type CDS
Coding Frame 1
Product Name
exotoxin A
Synonyms
Evidence for Translation
Charge (pH 7) -17.12
Kyte-Doolittle Hydrophobicity Value -0.263
Molecular Weight (kDa) 69.1
Isoelectric Point (pI) 5.21

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1XK9 TRANSFERASE 09/28/04 Pseudomanas exotoxin A in complex with the PJ34 inhibitor PSEUDOMONAS AERUGINOSA 2.1 X-RAY DIFFRACTION 98.6
1IKQ TRANSFERASE 05/04/01 Pseudomonas Aeruginosa Exotoxin A, wild type PSEUDOMONAS AERUGINOSA 1.62 X-RAY DIFFRACTION 98.7
1ZM2 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 3.07 X-RAY DIFFRACTION 99.0
2ZIT BIOSYNTHETIC PROTEIN/TRANSFERASE 02/24/08 Structure of the eEF2-ExoA-NAD+ complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 3.0 X-RAY DIFFRACTION 99.0
1ZM4 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of the eEF2-ETA-bTAD complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 2.9 X-RAY DIFFRACTION 99.0
1ZM3 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of the apo eEF2-ETA complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 3.07 X-RAY DIFFRACTION 99.0
1IKP TRANSFERASE 05/04/01 Pseudomonas Aeruginosa Exotoxin A, P201Q, W281A mutant PSEUDOMONAS AERUGINOSA 1.45 X-RAY DIFFRACTION 98.4
1ZM9 BIOSYNTHETIC PROTEIN/TRANSFERASE 05/10/05 Structure of eEF2-ETA in complex with PJ34 PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 2.8 X-RAY DIFFRACTION 99.0
1DMA ADP-RIBOSYLATION 04/28/95 DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH NICOTINAMIDE AND AMP PSEUDOMONAS AERUGINOSA 2.5 X-RAY DIFFRACTION 98.6
1AER ADP-RIBOSYLATION 12/11/95 DOMAIN III OF PSEUDOMONAS AERUGINOSA EXOTOXIN COMPLEXED WITH BETA-TAD PSEUDOMONAS AERUGINOSA 2.3 X-RAY DIFFRACTION 98.6
3B78 BIOSYNTHETIC PROTEIN/TRANSFERASE 10/30/07 Structure of the eEF2-ExoA(R551H)-NAD+ complex PSEUDOMONAS AERUGINOSA; SACCHAROMYCES CEREVISIAE 2.5 X-RAY DIFFRACTION 98.5

Virulence Evidence

Source 1
Database PseudoCAP
Category Exototoxin A
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Arabidopsis thaliana
Notes
Infection model Arabidopsis leaf infiltration assay
Reference 10922040
Source 2
Database Victors : 4410
Category Exototoxin A
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 7604262
Source 3
Database PseudoCAP
Category Exototoxin A
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Burned mouse model
Reference 10922040

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in one genus

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001108 (298 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_49560
Search term: toxA
Search term: exotoxin A

References

No references are associated with this feature.