Pseudomonas aeruginosa UCBPP-PA14, PA14_54640 (dspI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
Locus Tag
PA14_54640
Name
dspI
Synonym: dispersion inducer
Replicon chromosome
Genomic location 4844691 - 4845509 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 7 transposon mutants in orthologs
Comment

Cross-References

RefSeq YP_792540.1
GI 116048660
EC Number 4.2.1.17
Entrez 4385292
INSDC ABJ09896.1
NCBI Locus Tag PA14_54640
UniParc UPI00000C5134
UniProtKB Acc Q02HP6
UniProtKB ID Q02HP6_PSEAB
UniRef100 UniRef100_W0YNS2
UniRef50 UniRef50_A9ANK2
UniRef90 UniRef90_W6RIF3

Product

Feature Type CDS
Coding Frame 1
Product Name
DspI
Product Name Confidence: Class 1
Synonyms enoyl-CoA hydratase
Evidence for Translation
Charge (pH 7) -1.94
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 29.9
Isoelectric Point (pI) 6.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5WYD ISOMERASE 01/12/17 Structural of Pseudomonas aeruginosa DspI PSEUDOMONAS AERUGINOSA PAO1 2.101 X-RAY DIFFRACTION 99.6
5WYB ISOMERASE 01/12/17 Structure of Pseudomonas aeruginosa DspI PSEUDOMONAS AERUGINOSA PA14 2.25 X-RAY DIFFRACTION 99.6

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Caenorhabditis elegans
Notes
Infection model C. elegans slow killing assay.
Reference 22911607

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 508 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000719 (1195 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_54640
Search term: dspI
Search term: DspI

Human Homologs

Ensembl 94, assembly GRCh38.p12
AU RNA binding methylglutaconyl-CoA hydratase [Source:HGNC Symbol;Acc:HGNC:890]
E-value: 7.5e-27
Percent Identity: 32.1

References

PubMed ID: 29500457
Genome-wide identification of Pseudomonas aeruginosa virulence-related genes using a Caenorhabditis elegans infection model.
Feinbaum RL, Urbach JM, Liberati NT, Djonovic S, Adonizio A, Carvunis AR, Ausubel FM
PLoS Pathog. 2012 ;8(7):e1002813
PubMed ID: 22911607