Pseudomonas aeruginosa UCBPP-PA14, PA14_56240 (pykA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_56240
Name	pykA
Replicon	chromosome
Genomic location	5020400 - 5021851 (+ strand)
Transposon Mutants	2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs	2 transposon mutants in orthologs

Cross-References

RefSeq	YP_792674.1
GI	116052363
EC Number	2.7.1.40
Entrez	4382414
INSDC	ABJ13599.1
NCBI Locus Tag	PA14_56240
UniParc	UPI000039633E
UniProtKB Acc	Q02HB2
UniProtKB ID	Q02HB2_PSEAB
UniRef100	UniRef100_Q02HB2
UniRef50	UniRef50_P21599
UniRef90	UniRef90_Q02HB2

Product

Feature Type	CDS
Coding Frame	1
Product Name	pyruvate kinase
Synonyms
Evidence for Translation
Charge (pH 7)	-3.24
Kyte-Doolittle Hydrophobicity Value	-0.091
Molecular Weight (kDa)	52.3
Isoelectric Point (pI)	6.52

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
6QXL	TRANSFERASE	03/07/19	Crystal Structure of Pyruvate Kinase II (PykA) from Pseudomonas aeruginosa in complex with sodium malonate, magnesium and glucose-6-phosphate	Pseudomonas aeruginosa	2.43	X-RAY DIFFRACTION	99.8

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 614 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG003926 (537 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_56240 Search term: pykA Search term: pyruvate kinase

Human Homologs

Ensembl 110, assembly GRCh38.p14	ENSP00000456970 pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021] E-value: 4.1e-67 Percent Identity: 36.7
Ensembl 110, assembly GRCh38.p14	ENSP00000455736 pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021] E-value: 4.1e-67 Percent Identity: 36.7
Ensembl 110, assembly GRCh38.p14	ENSP00000455901 pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021] E-value: 4.1e-67 Percent Identity: 36.7
Ensembl 110, assembly GRCh38.p14	ENSP00000320171 pyruvate kinase M1/2 [Source:HGNC Symbol;Acc:HGNC:9021] E-value: 4.1e-67 Percent Identity: 36.7

References