Pseudomonas aeruginosa UCBPP-PA14, PA14_57425 (ftsI)

Gene Feature Overview

Strain	Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006) GCF_000014625.1|latest
Locus Tag	PA14_57425
Name	ftsI
Replicon	chromosome
Genomic location	5116162 - 5117901 (- strand)
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	YP_792764.1
GI	116054422
Entrez	4384195
INSDC	ABJ15658.1
NCBI Locus Tag	PA14_57425
UniParc	UPI00000D425F
UniProtKB Acc	Q02H22
UniProtKB ID	Q02H22_PSEAB
UniRef100	UniRef100_W0YZE8
UniRef50	UniRef50_H8W655
UniRef90	UniRef90_A0A0A8RLY9

Product

Feature Type	CDS
Coding Frame	1
Product Name	penicillin-binding protein 3
Synonyms
Evidence for Translation
Charge (pH 7)	16.14
Kyte-Doolittle Hydrophobicity Value	-0.125
Molecular Weight (kDa)	62.9
Isoelectric Point (pI)	10.04

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3OCN	PENICILLIN-BINDING PROTEIN/ANTIBIOTIC	08/10/10	Crystal structure of penicillin-binding protein 3 from Pseudomonas aeruginosa in complex with ceftazidime	Pseudomonas aeruginosa	2.61	X-RAY DIFFRACTION	100.0
5DF8	TRANSFERASE	08/26/15	CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH CEFOPERAZONE	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	100.0
4KQQ	Biosynthetic Protein/Antibiotic	05/15/13	CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH (5S)-Penicilloic Acid	Pseudomonas aeruginosa	2.1	X-RAY DIFFRACTION	100.0
6I1E	PEPTIDE BINDING PROTEIN	10/28/18	Crystal structure of Pseudomonas aeruginosa Penicillin-Binding Protein 3 in complex with amoxicillin	Pseudomonas aeruginosa	1.64	X-RAY DIFFRACTION	99.6
3OCL	PENICILLIN-BINDING PROTEIN/ANTIBIOTIC	08/10/10	Crystal structure of penicillin-binding protein 3 from Pseudomonas aeruginosa in complex with carbenicillin	Pseudomonas aeruginosa	2.3	X-RAY DIFFRACTION	100.0
6HR4	PENICILLIN-BINDING PROTEIN	09/26/18	Apo form of penicillin-binding protein 3 from P. aeruginosa	Pseudomonas aeruginosa PAO1	1.19	X-RAY DIFFRACTION	99.6
3OC2	PENICILLIN-BINDING PROTEIN	08/09/10	Crystal structure of penicillin-binding protein 3 from Pseudomonas aeruginosa	Pseudomonas aeruginosa	1.968	X-RAY DIFFRACTION	100.0
4KQR	Biosynthetic Protein/Antibiotic	05/15/13	CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH (5S)-Penicilloic Acid	Pseudomonas aeruginosa	2.01	X-RAY DIFFRACTION	100.0
7ATW	HYDROLASE	11/01/20	Structure of P. aeruginosa PBP3 in complex with a benzoxaborole (Compound 3)	Pseudomonas aeruginosa	1.44	X-RAY DIFFRACTION	99.6
7ATO	HYDROLASE	10/30/20	Structure of P. aeruginosa PBP3 in complex with an aryl boronic acid (Compound 2)	Pseudomonas aeruginosa PAO1	1.587	X-RAY DIFFRACTION	99.6
4KQO	Biosynthetic Protein/Antibiotic	05/15/13	Crystal structure of penicillin-binding protein 3 from pseudomonas aeruginosa in complex with piperacillin	Pseudomonas aeruginosa	2.31	X-RAY DIFFRACTION	100.0
7ATM	HYDROLASE	10/30/20	Structure of P. aeruginosa PBP3 in complex with a phenyl boronic acid (Compound 1)	Pseudomonas aeruginosa PAO1	1.582	X-RAY DIFFRACTION	99.6
7KIW	HYDROLASE/Inhibitor/Antibiotic	10/25/20	Crystal structure of Pseudomonas aeruginosa PBP3 in complex with zidebactam	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	2.49	X-RAY DIFFRACTION	100.0
7ATX	HYDROLASE	11/01/20	Structure of P. aeruginosa PBP3 in complex with a benzoxaborole (Compound 4)	Pseudomonas aeruginosa	1.795	X-RAY DIFFRACTION	99.6
5DF7	TRANSFERASE	08/26/15	CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH AZLOCILLIN	Pseudomonas aeruginosa	2	X-RAY DIFFRACTION	100.0
8BH1	MEMBRANE PROTEIN	10/28/22	Core divisome complex FtsWIQBL from Pseudomonas aeruginosa	Pseudomonas aeruginosa PAO1	3.8	ELECTRON MICROSCOPY	100.0
5DF9	TRANSFERASE	08/26/15	CRYSTAL STRUCTURE OF PENICILLIN-BINDING PROTEIN 3 IN COMPLEX WITH DEACYLATED PRODUCT OF CEFOPERAZONE	Pseudomonas aeruginosa	2.7	X-RAY DIFFRACTION	100.0
7KIV	HYDROLASE/Inhibitor/Antibiotic	10/24/20	Crystal structure of Pseudomonas aeruginosa PBP3 in complex with avibactam	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	2.389	X-RAY DIFFRACTION	100.0
7KIT	HYDROLASE/Inhibitor/Antibiotic	10/24/20	Crystal structure of Pseudomonas aeruginosa PBP3 in complex with WCK 4234	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	2.089	X-RAY DIFFRACTION	100.0
6HR6	PENICILLIN-BINDING PROTEIN	09/26/18	Nitrocefin reacted with catalytic serine (Ser294) of penicillin-binding protein 3 from Pseudomonas aeruginosa	Pseudomonas aeruginosa	2.53	X-RAY DIFFRACTION	99.6

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name	Source Accession	Source DB	Version	Target Accession	Target Description	Percent Identity	Alignment Length	E-Value
Cloxacillin	DB01147	DrugBank	5.1.4	Q51504	Cell division protein	100.0	579	0.0e+00
Ceftolozane	DB09050	DrugBank	5.1.4	Q51504	Cell division protein	100.0	579	0.0e+00
Cefepime	DB01413	DrugBank	5.1.4	Q51504	Cell division protein	100.0	579	0.0e+00
Doripenem	DB06211	DrugBank	5.1.4	Q51504	Cell division protein	100.0	579	0.0e+00

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 575 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG004008 (535 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA14_57425 Search term: ftsI Search term: penicillin-binding protein 3

Human Homologs

References