Pseudomonas aeruginosa UCBPP-PA14, PA14_60970 (mapZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse

Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
GCF_000014625.1|latest
Locus Tag
PA14_60970
Name
mapZ
Replicon chromosome
Genomic location 5439358 - 5439735 (+ strand)
Transposon Mutants 1 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq YP_793067.1
GI 116052750
Entrez 4384582
INSDC ABJ13986.1
NCBI Locus Tag PA14_60970
UniParc UPI00000C5D87
UniProtKB Acc Q02G69
UniProtKB ID Q02G69_PSEAB
UniRef100 UniRef100_Q9HVI1
UniRef50 UniRef50_Q9HVI1
UniRef90 UniRef90_Q9HVI1

Product

Feature Type CDS
Coding Frame 1
Product Name
MapZ
Product Name Confidence: Class 1
Synonyms methyltransferase-associated PilZ
Evidence for Translation
Charge (pH 7) -11.35
Kyte-Doolittle Hydrophobicity Value -0.377
Molecular Weight (kDa) 14.6
Isoelectric Point (pI) 4.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5Y4R TRANSFERASE/PROTEIN BINDING 08/04/17 Structure of a methyltransferase complex Pseudomonas aeruginosa str. PAO1 2.298 X-RAY DIFFRACTION 100.0
5XLY TRANSFERASE 05/12/17 Crystal structure of CheR1 in complex with c-di-GMP-bound MapZ Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.763 X-RAY DIFFRACTION 100.0
1YWU STRUCTURAL GENOMICS, UNKNOWN FUNCTION 02/18/05 Solution NMR structure of Pseudomonas Aeruginosa protein PA4608. Northeast Structural Genomics target PaT7 Pseudomonas aeruginosa NOT SOLUTION NMR 100.0
2L74 c-di-GMP binding protein 12/02/10 Solution structure of the PilZ domain protein PA4608 complex with c-di-GMP identifies charge clustering as molecular readout Pseudomonas aeruginosa NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 39 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004263 (504 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_60970
Search term: mapZ
Search term: MapZ

Human Homologs

References

A cyclic di-GMP-binding adaptor protein interacts with a chemotaxis methyltransferase to control flagellar motor switching.
Xu L, Xin L, Zeng Y, Yam JK, Ding Y, Venkataramani P, Cheang QW, Yang X, Tang X, Zhang LH, Chiam KH, Yang L, Liang ZX
Sci Signal 2016 Oct 18;9(450):ra102
PubMed ID: 27811183
The MapZ-Mediated Methylation of Chemoreceptors Contributes to Pathogenicity of Pseudomonas aeruginosa.
Sheng S, Xin L, Yam JKH, Salido MM, Khong NZJ, Liu Q, Chea RA, Li HY, Yang L, Liang ZX, Xu L
Front Microbiol 2019;10:67
PubMed ID: 30804897
Solution structure of the PilZ domain protein PA4608 complex with cyclic di-GMP identifies charge clustering as molecular readout.
Habazettl J, Allan MG, Jenal U, Grzesiek S
J Biol Chem 2011 Apr 22;286(16):14304-14
PubMed ID: 21310957